Quality Standard of Yi Herb Medicine Millettia dielsiana

[Objectives]The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana.[Methods]Yi herb medicine M.dielsiana was qualitatively identified by microscopic identification and thin layer identification.The content of total isoflavones was determined by UV-Vis spectrophotometry,and the contents of moisture,total ash,acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia(2020 edition).[Results]The microscopic identification features,including stone cells,fiber bundles,calcium oxalate square crystals,starch granules,pigment blocks,ducts,etc.,were distinct.A TLC method for identification of M.dielsiana was established.The contents of water,total ash,acid-insoluble ash and extract should be no more than 13%,no more than 7%,no more than 0.5%and no less than 6%in 10 batches of samples,respectively.The total isoflavones of M.dielsiana based on genistein(C_(15)H_(10)O_(5)) should be no less than 0.25%.The linear relationship of genistein was good in the range of 0.002-0.007 mg/mL(R^(2)=0.9996);the average recovery was 97.01%and the RSD was 2.62%.[Conclusions]The method is simple,accurate and well-reproducible,and can be used for the quality control of Yi herb medicine M.dielsiana.