阿尔茨海默病与microRNA⁃206基因多态性的关系

Association between microRNA⁃206 gene polymorphisms and Alzheimer′s disease

目的分析阿尔茨海默病与microRNA⁃206基因多态性的关系。方法采用SNaPshot技术对100例阿尔茨海默病患者(病例组)和100例健康体检者(对照组)的microRNA⁃206基因rs16882131位点进行基因型检测,采用荧光定量多重聚合酶链反应方法检测两组外周血microRNA⁃206表达水平,并分析其与rs16882131位点不同基因型的关系。结果病例组rs16882131位点的基因型和等位基因频率分布与对照组比较,差异均有统计学意义(基因型CC vs.CT vs.TT:P=0.027,TT+CT vs.CC:P=0.034,OR=1.831;等位基因T vs.C:P=0.005,OR=1.860);病例组外周血microRNA⁃206表达水平明显高于对照组(P<0.05);对照组TT+CT基因型(T等位基因携带)的microRNA⁃206表达水平明显高于CC基因型(P<0.05)。结论microRNA⁃206基因rs16882131位点多态性与阿尔茨海默病易感性有关,T等位基因可能是阿尔茨海默病发病的危险因素。

Objective To analyze the relationship of microRNA⁃206 gene polymorphisms and Alzheimer′s disease(AD).Methods The SNP rs16882131 in the microRNA⁃206 was genotyped in 100 AD patients and 100 healthy controls using the SNaPshot technique.Quantitative real⁃time PCR was employed to evaluate the levels of microRNA⁃206 expression in the peripheral blood of the two groups,and the association of the SNP rs16882131 with microRNA⁃206 expression was further analyzed.Results There were significant differences in the genotype and allele frequencies of the SNP rs16882131 between the AD patients and controls(genotype CC vs.CT vs.TT:P=0.027,TT+CT vs.CC:P=0.034,OR=1.831;allele T vs.C:P=0.005,OR=1.860).The microRNA⁃206 expression in the peripheral blood of the AD patients was significantly higher than that in the healthy controls(P<0.05).Additionally,the microRNA⁃206 expression in the healthy controls who were TT+CT genotypes of the SNP rs16882131 was significantly higher compared with the CC genotypes(P<0.05).Conclusion The SNP rs16882131 in the microRNA⁃206 is associated with the susceptibility to the AD,and the T allele may contribute to risk roles in the AD.