固定化青霉菌脱色亚甲基蓝

Decolorization of methylene blue by immobilized Penicillium

研究大豆分离蛋白和海藻酸钠微胶囊固定化青霉菌实验,确定最佳条件为m(大豆分离蛋白)∶m(海藻酸钠微胶囊)=1∶1,pH=3。在扫描电镜下观察,发现该微胶囊具有疏松多孔结构。单一的固定化材料大豆分离蛋白和海藻酸钠对亚甲基蓝的脱色效率分别为4.01%和1.71%(24 h),而包裹青霉菌的大豆分离蛋白-海藻酸钠微胶囊样品,24 h后亚甲基蓝的脱色效率为94.21%,合成后的微胶囊结构对亚甲基蓝具有吸附作用。考察探究大豆分离蛋白微胶囊固定化青霉菌在不同的环境条件下脱色亚甲基蓝的脱色效率,青霉菌经过固定化后,抵抗强酸、强碱和高浓度亚甲基蓝浓度的能力较强。

The immobilization of Penicillium spp.with soybean protein isolate and sodium alginate microcapsules was studied.The optimum conditions were determined as follows: m(soybean protein isolate)∶m(sodium alginate microcapsules)=1∶1,pH=3.Under scanning electron microscope, it was found that the microcapsule had loose and porous structure.The decolorization efficiencies of soybean protein isolate and sodium alginate for methylene blue were 4.01% and 1.71%,respectively, while the decolorization efficiency of soybean protein isolate sodium alginate microcapsule wrapped with Penicillium was 94.21% after 24 hours.It can be seen that the synthesized microcapsule structure has adsorption ability on methylene blue.The decolorization efficiency of soybean protein isolate microcapsule immobilized Penicillium under different environmental conditions was investigated.It was found that the ability of Penicillium to resist strong acid, strong alkali and high concentration of methylene blue was improved after immobilization.