基于线粒体DNA tRNA^(leu)-COⅡ序列的浙江省东方蜜蜂遗传多样性研究

Genetic diversity of Apis cerana in Zhejiang,China based on mitochondrial DNA tRNA^(leu)-COⅡsequence

为进一步了解浙江省东方蜜蜂的遗传多样性状况,基于浙江省10个地级市的448群东方蜜蜂样本,利用线粒体DNA tRNA^(leu)-COⅡ序列进行遗传变异与群体遗传结构分析。结果显示,共获得46个单倍型,单倍型多样度为0.6657,核苷酸多样度为0.00261,表明浙江省东方蜜蜂具有较高的单倍型多样度、较低的核苷酸多样度。中性检验和单倍型网络图分析显示,浙江省东方蜜蜂可能经历了群体扩张事件。群体遗传结构分析显示,杭州、宁波与周边其他地区存在一定程度的遗传分化,可能与东方蜜蜂在当地的长期生存适应,以及一定的地理隔离有关。研究还新发现一个与国内其他地区东方蜜蜂差异较大的特殊单倍型。

To further understand the genetic diversity of Apis cerana in Zhejiang Province,the genetic variation and population genetic structure were determined based on the mitochondrial DNA tRNA^(leu)-COⅡsequences of 448 samples from ten prefecture-level cities in Zhejiang,China.Forty-six haplotypes were found and the haplotype diversity(H_(d))was 0.6657,the nucleotide diversity(P_(i))was 0.00261,which indicated that A.cerana in Zhejiang Province had relatively high haplotype diversity and low nucleotide diversity.Neutral test and network of haplotypes showed that A.cerana in Zhejiang Province might have experienced recent expansion.Population genetic structure analysis showed that A.cerana populations in Hangzhou and Ningbo had a certain extent of genetic differentiations with other surrounding A.cerana populations,which was possibly related to the survivorship and adaptation of A.cerana in the local areas over a long period of time and a certain geographical isolation.A new haplotype that highly differentiated with other haplotypes of A.cerana in China was found in this study.