浙江省地方菜豆种质资源鉴定与遗传多样性分析

Evaluation and genetic diversity analysis of common bean germplasm in Zhejiang Province,China

为鉴定浙江省地方菜豆种质遗传多样性,对浙江省历年来收集到的44份菜豆种质进行13个重要农艺性状的田间调查。结果表明,浙江地方菜豆种质在生长习性、嫩荚商品性等性状上均存在丰富的遗传变异。从发表的菜豆BARCBean6K_3 BeadChip芯片中随机、均匀挑选了185个SNP转化成KASP标记,对44份种质进行基因型鉴定后获得了88个高质量SNP基因型,主成分分析和系统进化树分析发现这些种质可以分为2大类,亚群分化与千粒重高度相关,且浙江省菜豆种质以中美洲基因池为主。研究结果可进一步丰富我国菜豆遗传基础,为地方菜豆资源的高效利用和新品种选育提供科学依据,所开发的KASP标记也可为菜豆种质基础研究提供有力的遗传工具。

To identify the genetic diversity of common bean germplasm in Zhejiang Province,44 common bean accessions collected from Zhejiang Province in multi-years were evaluated on 13 basic agronomic traits in the field,and it is found that large genetic variation presented on the traits including the growth habits,fresh pod commerciality in this collection.In addition,185 SNPs from the known BARCBean6K_3 BeadChip were randomly selected and converted into KASP markers,finally the high quality genotypes of the 44 accessions were obtained from 88 KASP-SNPs.Based on the genotype data of this population,PCA and phylogenetic tree analysis both showed that Zhejiang common bean germplasms could be divided into two subpopulations,and the subpopulations differentiation was strongly associated with thousand seed weight,and most of Zhejiang common bean germplasm belong to Mesoamerican gene pool.The study results will further enrich the genetic basis of Chinese common bean germplasms and provide a scientific basis for efficient use of local resources and breeding new cultivars.