不同产地三叶木通果胶的理化性质、抗氧化活性及对癌细胞A549和K562-2生长的抑制作用

Physicochemical properties,antioxidant activities,and inhibitory capabilities of pectins in the pericarps of Akebia trifoliata from different regions on cancer cells A549 and K562-2

为探明不同产地三叶木通果胶的理化性质及生物活性的差异,该研究采用酸提醇沉法对来源于贵州凯里及河南南阳的三叶木通果皮果胶进行提取,比较两者之间理化性质、清除羟自由基(·OH)和DPPH自由基能力及对人肺癌细胞(A549)和人慢性髓系白血病细胞(K562-2)抑制效果。结果表明,酸提醇沉法提取的两产地三叶木通果胶均为高酯果胶,其半乳糖醛酸的含量及酯化度均高于商品果胶,对·OH、DPPH自由基均有一定清除作用;在低质量浓度时(≤0.5 mg/mL),两产地果胶清除·OH能力无显著差异,随着果胶液浓度的增大,河南南阳果胶清除·OH及DPPH自由基能力显著高于贵州凯里果胶。MTT法测试结果显示,两产地果胶对癌细胞珠A549和K562-2的生长均有一定的抑制作用,贵州凯里果胶对A549和K562-2的抑制率略高于河南南阳果胶。三叶木通果皮果胶在医药及保健方面具有更好的开发前景,该研究为不同产地的三叶木通开发利用提供一定的理论依据。

To find out the differences in physicochemical properties and biological activities of Akebia trifoliata pectin from different regions,the pectins in the peel of Akebia trifoliata from two regions(Kaili,Guizhou and Nanyang,Henan)were extracted using acid ex-traction and alcohol precipitation.The physicochemical properties,the ability to scavenge·OH and DPPH free radicals,and the inhibi-tory effect on human lung cancer cells(A549)and human chronic myeloid leukemia cells(K562-2)were compared between these two ex-tracted pectins.It was found that the pectin extracted by acid extraction and alcohol precipitation from both regions belonged to the cate-gory of high ester pectin,and its galacturonic acid content and esterification degree were higher than that of commercial pectin,which had a certain scavenging effect on·OH and DPPH free radicals.At low concentrations(≤0.5 mg/mL),there was no significant difference in the ability of these two pectins to remove·OH from the two regions.However,as the pectin concentration increased(>0.5 mg/mL),the scavenging capacity of Nanyang pectin in Henan province was significantly higher than that of Kaili pectin in Guizhou province.The results by MTT showed that the pectin from both producing areas had the inhibitory ability on the growth of cancer cells A549 and K562-2.The inhibitory rate of Kaili pectin on A549 and K562-2 was slightly higher than that of Nanyang pectin.This study reveals that the peel pectin of Akebia trifoliata has a better development prospect in medicine and health care and provides a certain theoretical basis for the development and utilization of Akebia trifoliata from different areas.