基于高通量测序解析浓香型大曲真菌群落结构及其与质量指标的关联性分析

Analysis of fungal community structure based on high-throughput sequencing and its correlation with quality indicators of strong-flavor Daqu

为明确不同地区浓香型大曲的真菌群落结构及其与质量指标的关联性,采用MiSeq高通量测序技术解析不同地区大曲曲皮与曲心的真菌群落结构,再分别检测其糖化力等7项质量指标,并进行真菌群落及其与质量指标的Spearman相关性分析。结果表明,不同地区浓香型大曲的曲皮共有真菌OTU为43个、曲心共有真菌OTU为47个,曲皮中的根霉(Rhizopus)、根毛霉(Rhizomucor)、布氏白粉菌(Blumeria)和散囊菌(Eurotium)为优势菌属,曲心中的嗜热菌(Thermomyces),散囊菌(Eurotium)和嗜热子囊菌(Thermoascus)为优势菌属,其丰度占比均在10%以上。相关性分析表明,嗜热菌(Thermomyces)与水分呈极显著正相关,被孢霉(Mortierella)与还原糖呈极显著正相关,根霉(Rhizopus)、匍柄霉(Stamphylium)和链格孢属(Alternaria)与糖化力呈显著正相关,罗萨氏菌(Rasamsonia)与酯化力呈显著正相关。该研究对大曲微生物组信息扩充具有一定的参考价值。

In order to clarify the fungal community structure of strong-flavor Daqu from different regions and its correlation with quali-ty indicators,MiSeq high-throughput sequencing was used to analyze the fungal community structure in surface and inner parts of Daqu from different regions.Saccharification capacity and other quality indicators were detected,and Spearman correlation analysis of fungal community and its quality indicators was taken.The results showed there were 43 fungal operational taxonomic units(OTUs)in common between surface samples,47 fungal OTUs in inner samples.Rhizopus,Rhizomucor,Blumeria,and Eurotium were the dominant genera in surface samples.While,Thermomyces,Eurotium,and Thermoascus were the dominant genera in inner samples,and their abundances were all account for more than 10%.The correlation analysis showed that there was a significant positive correlation between Thermomyces and moisture,Mortierella and reducing sugar.Rhizopus,Stamphylium and Alternaria were significantly positively correlated with sacchari-fication capacity,and Rasamsonia was significantly positively correlated with esterification capacity.This study has certain reference value for the expansion of Daqu microbiome information.