广谱性抗体的制备及其在食品安全快速检测中的应用进展

The preparation of broad-spectrum antibodies and their application in the rapid detection of food safety

“民以食为天,食以安为先”,在追求效益的时代背景下,危害物的高通量高效率检测成为食品安全快速检测领域发展的新趋势。基于广谱性抗体的免疫检测法凭借其检测通量高、检测时间短、样品消耗少、总成本低等优点为多危害物同时快速检测提供了一种新思路。该文指出了广谱性抗体的3种主要制备方法及优缺点,分析了提高广谱性抗体广谱性及亲和力的常用策略,并对其在毒素类、农兽药类残留及其他常见食品危害物检测中的应用现状进行综述。该文对广谱性抗体目前存在的制备盲目性和检测灵敏性较差两大主要限制因素进行总结分析,并展望了广谱性抗体在食品安全领域的发展趋势。

As an old saying goes,food is the paramount necessity of people and food safety is the top priority.In the era of pursuing efficiency,the high-throughput and high-efficiency detection of hazards has become a new trend in the rapid detection of food safety.The immunoassay based on broad-spectrum antibodies provides a new idea for simultaneous and rapid detection of multiple hazards with its ad-vantages of high detection flux,short detection time,less sample consumption,and low total cost.This paper pointed out three main prep-aration methods of broad-spectrum antibodies and their relative merits,analyzed the common strategies to improve the cross-reactivity and affinity of broad-spectrum antibodies,and reviewed their application in the detection of toxins,residues of agricultural and veterinary drugs,and other common food hazards.At the same time,this paper summarized the two main limiting factors of broad-spectrum antibod-ies:preparation blindness and poor detection sensitivity,and finally,looked forward to the development trend of broad-spectrum antibod-ies in the field of food safety.