海参废渣皂苷通过核苷酸结合寡聚化结构域样受体信号通路抵抗环磷酰胺诱导小鼠的免疫抑制

Saponins from Sea Cucumber Residues Protect against Cyclophosphamide Induced Immunosuppression in Mice via the Nucleotide Binding and Oligomerization Domain-Like Receptor Signaling Pathway

在我国海参是一种具有多种生物活性的传统滋补品。本实验系统性地研究了2.5、5.0、10.0 mg/(kg m_(b)·d)海参废渣皂苷(saponins extracted from sea cucumber residues,SCRSS)对免疫抑制小鼠的调节作用。结果表明,与模型对照组相比,5.0 mg/(kg m_(b)·d)SCRSS使小鼠的体质量、脾脏指数和胸腺指数分别增加了15.3%、23.8%和39.8%;5.0 mg/(kg m_(b)·d)SCRSS也极显著增加了免疫抑制小鼠的吞噬指数(P<0.01)。此外,与模型对照组相比,各剂量SCRSS均明显地增加了血清中细胞因子的水平,并促进了脾淋巴细胞和腹腔巨噬细胞的增殖活性。其中,中等剂量(5.0 mg/(kg m_(b)·d))SCRSS可以更有效地上调免疫抑制小鼠核苷酸结合寡聚化结构域蛋白1、核苷酸结合寡聚化结构域蛋白2、受体相互作用蛋白-2和核因子-κB的表达。结论:SCRSS具有作为提高免疫力的功能性食品补充剂的潜力。

Sea cucumber is a traditional Chinese tonic with various bioactivities.This study systematically investigated the immunomodulatory effect of saponins extracted from sea cucumber residues(SCRSS)at doses of 2.5,5.0 and 10.0 mg/(kg m_(b)·d)on immunosuppressive mice.The results showed that SCRSS at a dose of 5.0 mg/(kg m_(b)·d)increased the body mass,spleen index,and thymus index by 15.3%,23.8%,and 39.8%,respectively,compared to the model control group,and it also significantly increased the phagocytic index in immunosuppressive mice(P<0.01).Moreover,All the doses of SCRSS remarkably increased serum cytokine levels,promoted the proliferation activity of splenic lymphocytes and peritoneal macrophages.Importantly,the middle dose(5.0 mg/(kg m_(b)·d))of SCRSS was more effective to upregulate the expression of nucleotide binding and oligomerization domain(NOD)1,NOD2,receptor interacting protein 2(RIP2)and nuclear factor(NF)-κB in immunosuppressive mice.Thus,SCRSS may be used as a functional food supplement to improve immunity.