铁皮石斛叶多糖对秀丽隐杆线虫体内抗衰老作用

Anti-aging Effect of Polysaccharide from Dendrobium officinale Leaves in Caenorhabditis elegans

目的:基于模式生物秀丽隐杆线虫(以下简称线虫)探究铁皮石斛叶多糖的抗衰老能力。方法:采用含不同质量浓度(0.5、1.0、2.0 mg/mL)铁皮石斛叶多糖的大肠埃希氏菌OP50菌液每天饲喂线虫,以饲喂大肠埃希氏菌OP50菌液组作为空白对照,观察铁皮石斛叶多糖对线虫寿命、生殖能力、运动能力和应激能力的影响,同时测定线虫体内活性氧水平及抗氧化酶的酶活力,分析铁皮石斛叶多糖对线虫体内抗氧化能力的影响。结果:与空白对照组线虫相比,在本实验考察的质量浓度范围内,铁皮石斛叶多糖均可延长线虫的寿命,提高其运动、应激能力,同时不会损害线虫的生殖能力,并且能有效提高过氧化氢酶和超氧化物歧化酶的活力,显著降低线虫体内丙二醛含量(P<0.05)。结论:铁皮石斛叶多糖可能通过提高线虫抗氧化酶活力及提高线虫抗应激能力,降低线虫体内活性氧水平并减缓细胞氧化损伤,进而发挥延长线虫寿命的功效。

Objective:To investigate the anti-aging activity of polysaccharide from Dendrobium officinale leaves in Caenorhabditis elegans.Methods:The suspension of Escherichia coli OP50 containing polysaccharide at different concentrations(0.5,1.0 and 2.0 mg/mL)was given to the nematode on a daily basis.A suspension of Escherichia coli OP50 was used as a control to evaluate the effects of the polysaccharide on the lifespan,reproductive capacity,locomotion behavior,and stress resistance of C.elegans.Meanwhile,the effects of the polysaccharide on antioxidant function in C.elegans were investigated by measuring the accumulation level of reactive oxygen species(ROS)and antioxidant enzyme activities.Results:Within the tested concentration range,the polysaccharide prolonged the lifespan,increased locomotion capacity and stress resistance of C.elegans without damaging its reproductive capacity,increased the activity of catalase,superoxide dismutase and significantly reduced the content of malondialdehyde(MDA)(P<0.05).The results showed that the polysaccharide from D.officinale leaves improves the activity of antioxidant enzymes and stress resistance,reduces ROS levels and alleviates cell oxidative damage,thereby prolonging the lifespan of C.elegans.