多囊卵巢综合征患者血清miR-143-3p、miR-222-3p及抗苗勒管激素水平检测及其临床价值

Detection and clinical value of serum levels of miR-143-3p,miR-222-3p and AMH in patients with polycystic ovary syndrome

目的探讨血清miR-143-3p、miR-222-3p水平联合抗苗勒管激素(AMH)在多囊卵巢综合征(PCOS)中的表达及临床价值。方法选取2018年1月—2022年3月三亚市妇女儿童医院收治的128例PCOS患者(PCOS组)和50例健康女性(对照组)。PCOS患者根据胰岛素抵抗指数(HOMA-IR)分为胰岛素抵抗组(83例,HOMA-IR≥2.69)和非胰岛素抵抗组(45例,HOMA-IR<2.69);根据BMI分为肥胖组(40例,BMI≥28)和非肥胖组(88例,BMI<28);根据睾酮水平分为高雄激素组(71例,睾酮≥0.7 ng/mL)及非高雄激素组(57例,睾酮<0.7 ng/mL)。采用实时定量PCR法检测血清miR-143-3p及miR-222-3p水平和电化学发光法检测AMH水平。ROC曲线分析miR-143-3p、miR-222-3p联合AMH对PCOS的诊断价值。相关性分析采用Pearson相关分析。结果PCOS组血清miR-143-3p、miR-222-3p及AMH水平均明显高于对照组,肥胖组明显高于非肥胖组,高雄激素组明显高于非高雄激素组,胰岛素抵抗组明显高于非胰岛素抵抗组,差异均有统计学意义(均P<0.001)。ROC曲线显示,miR-143-3p、miR-222-3p及AMH三项联合诊断PCOS的曲线下面积最大(AUC=0.910,95%CI:0.852~0.974)。PCOS患者血清miR-143-3p、miR-222-3p水平与AMH均呈正相关关系(r=0.842、0.795,均P<0.001)。结论PCOS患者血清miR-143-3p、miR-222-3p及AMH水平明显升高,三项联合检测对PCOS诊断具有较高的价值。

Objective To investigate the expression and clinical value of serum miR-143-3p,miR-222-3p levels combined with anti-Mullerian hormone(AMH)in polycystic ovary syndrome(PCOS).Methods Total 128 patients with PCOS(PCOS group)treated in Sanya Women and Children's Hospital from January 2018 to March 2022 and 50 healthy women(control group)were selected.According to insulin resistance index(HOMA-IR),PCOS patients were divided into insulin resistance group(n=83,HOMA-IR≥2.69)and non-insulin resistance group(n=45,HOMA-IR<2.69).According to BMI,PCOS patients were divided into obesity group(n=40,BMI≥28)and non-obesity group(n=88,BMI<28).According to the level of testosterone,they were divided into hyperandrogen group(n=71,testosterone≥0.7 ng/mL)and non-hyperandrogen group(n=57,testosterone<0.7 ng/mL).The serum levels of miR-143-3p and miR-222-3p were detected by real-time quantitative PCR,and AMH was detected by electrochemiluminescence.The diagnostic value of miR-143-3p,miR-222-3p combined with AMH in PCOS were analyzed by receiver operating characteristic(ROC)curve.Pearson correlation analysis was used for correlation analysis.Results The serum levels of miR-143-3p,miR-222-3p and AMH in PCOS group were significantly higher than those in control group,those in obesity group was significantly higher than that in non-obesity group,those in hyperandrogen group was significantly higher than that in non-hyperandrogen group,and insulin resistance group was significantly higher than that in non-insulin resistance group,with statistical significance(all P<0.001).ROC curve showed that the combined diagnosis of PCOS by miR-143-3p,miR-222-3p and AMH had the largest area under the curve(AUC=0.910,95%CI:0.852-0.974).The serum levels of miR-143-3p and miR-222-3p in PCOS patients were positively correlated with AMH(r=0.842,0.795,all P<0.001).Conclusion The serum levels of miR-143-3p,miR-222-3p and AMH were significantly increased in PCOS patients,and the detection of the three items were helpful for the diagnosis of PCOS.