探讨SOAT1联合多种标记物在肝细胞性肝癌辅助诊断中的应用

Application of SOAT1 combined with multiple markers in the auxiliary diagnosis of hepatocellular carcinoma

目的:探究甾醇氧-酰基转移酶1(SOAT1)在肝细胞性肝癌组织中的表达情况,并探讨其联合多种标记物(HSP70、GS、CD10、CD34)在诊断肝细胞性肝癌中的应用可行性。方法:收集58例肝细胞性肝癌组织、配对癌旁肝组织及12例良性肝病变组织样本,制作组织芯片,免疫组织化学方法检测SOAT1、HSP70、GS、CD10、CD34的表达情况,并分析其表达相关性。结果:SOAT1在肝细胞性肝癌组织中表达上调(P<0.01),且表达水平与肝细胞性肝癌患者分化程度密切相关(P<0.05)。SOAT1与HSP70、GS、CD34表达相关(P<0.01);肝细胞性肝癌诊断阳性符合率分别为CD34100.00%、GS 89.66%、HSP7082.75%、CD1063.79%、SOAT163.79%;而在癌旁组织中诊断阴性符合率分别为SOAT1100.00%、CD1098.27%、HSP7096.55%、CD3434.48%、GS 5.17%;良性肝病变组织中诊断阴性符合率分别为SOAT1100.00%、CD1083.34%、HSP70100.00%、CD348.33%、GS 0%。以任意3种标记物阳性为诊断标准,不将SOAT1计入标记物行列,58例肝细胞性肝癌中有39例可被诊断,诊断率为67.24%;而将SOAT1计入标记物行列,58例肝细胞性肝癌中有48例可被诊断,诊断率可提高至82.76%。结论:SOAT1在肝细胞性肝癌中表达水平明显上调,且在低分化肝细胞性肝癌表达水平更高,提示SOAT1可作为肝细胞性肝癌的辅助诊断及预后判断指标之一。SOAT1、HSP70、GS、CD10及CD345种标记物中,敏感性较好为CD34及GS,而特异性较好则为SOAT1、CD10及HSP70。SOAT1与HSP70、GS、CD10、CD34联合应用,在肝细胞性肝癌的辅助诊断中有一定应用价值。

Objective:To explore the expression of sterol oxygen-acyltransferase 1(SOAT1)in hepatocellular carcinoma tissues and to evaluate the application value of its combination with various markers(HSP70,GS,CD10,CD34)in the diagnosis of hepatocellular carcinoma.Methods:Totally 58 cases of liver cancer tissue and its corresponding adjacent tissue,and 12 cases of benign liver lesions tissue were collected,and tissue chips were made to detect the expressions of SOAT1,HSP70,GS,CD10and CD34(immunohistochemical staining method)which were analyzed by scientific method.Results:The expression of SOAT1was up-regulated in hepatocellular carcinoma tissues,and the difference was statistically significant(P<0.01),and the expression level was closely related to the differentiation degree of hepatocellular carcinoma patients(P<0.05).SOAT1 was correlated with the expression of HSP70,GS,and CD34,with statistical significance(P<0.01).Positive percent agreement for the hepatocellular carcinoma was 100.00%for CD34,89.66%for GS,82.75%for HSP70,63.79%for CD10,and 63.79%for SOAT1;negative percent agreement(paracancerous tissues)was 100%for SOAT1,98.27%for CD10,96.55%for HSP70,34.48%for CD34,and 5.17%for GS;Negative percent agreement(benign tissues)was 100%for SOAT1,83.34%for CD10,100.00%for HSP70,8.33%for CD34,and 0%for GS,respectively.Taking the positive expression of any three markers as the diagnostic criteria,39 of the 58 cases of hepatocellular carcinoma could be diagnosed,and the diagnostic rate was 67.24%(SOAT1 was not included in the list of markers).If SOAT1 is included,48 of the 58 hepatocellular carcinoma cases could be diagnosed,and the diagnosis rate could be increased to 82.76%.Conclusions:The expression level of SOAT1 was significantly up-regulated in hepatocellular carcinoma,and the expression level was higher in poorly differentiated HCC,suggesting that SOAT1 can be used as one of the indicators for the diagnosis and prognosis of hepatocellular carcinoma.Among the five markers above,CD34 and GS were more sensitive,while the specificity is better for SOAT1,CD10 and HSP70.The combined application of SOAT1 with HSP70,GS,CD10 and CD34 has certain application value in the diagnosis of hepatocellular carcinoma.