盐度对香螺行为、鳃和肾抗氧化酶活性与组织结构的影响

Effect of salinity on Neptunea cumingii behavior and antioxi-dant enzyme activities and tissue structure of the gill and kidney

为明确不同盐度胁迫下香螺的生理生态学响应及其适应机制,本文采用行为学、组织学和生物化学手段研究了不同盐度条件下(16、20、24、28、32、36、40、44、48)香螺的行为、病理损伤以及抗氧化酶活性。结果显示,在高盐和低盐胁迫下,香螺出现应激反应,摄食率下降,死亡率升高。随盐度升高,香螺的总抗氧化能力先升高后降低,在盐度32时达到峰值;香螺鳃的过氧化氢酶(CAT)活力和超氧化物歧化酶(SOD)活力呈先降低后升高的趋势;肾的CAT和SOD活力呈先升高后降低的趋势。组织切片结果显示,在高盐和低盐胁迫下,香螺嗅检器和鳃丝的形态呈现显著变化,黏液细胞数量均增加。上述结果表明,盐度显著影响香螺的生理生态反应,且香螺可通过行为变化、调节抗氧化酶以及改变组织形态结构从而适应外界盐度的变化。

The behavior,pathological damage,and antioxidant enzyme activities of Neptunea cumingii under dif-ferent salinity conditions(16,20,24,28,32,36,40,44,and 48)were studied using different methods to clarify the physiological and ecological response and adaptive mechanism of N.cumingii under different salinities.The results showed that N.cumingii exhibited a stress response,decreased feeding rate,and increased mortality under high and low salt stress.The total antioxidant capacity of N.cumingii first increased and then decreased as salinity increased from 16 to 32.The activities of catalase(CAT)and superoxide dismutase(SOD)in gill first decreased and then increased.Kidney CAT and SOD activities first increased and then decreased.The tissue section results showed that the morphology of the N.cumingii olfactory apparatus and gill filaments changed significantly under high salt and low salt stress,and the number of mucus cells increased.These results show that salinity significantly affected the physiological and ecological responses of N.cumingii.N.cumingii adapted to the external salinity changes by changing behavior,regulating antioxidant enzyme activities,and changing tissue morphological structure.