摘要
以猴头菇纯化多糖(Hericium erinaceus polysaccharide,HEPS)(总糖含量>92%)为研究对象,在含有VC和亚硒酸钠的氧化还原体系中,制备得到猴头菇多糖纳米硒复合物(HEPS-Se)。通过原子吸收分光光谱仪、傅里叶变换红外光谱仪、X射线衍射仪、扫描电子显微镜、EDX元素分析仪、X射线光电子能谱、纳米粒度仪等对其结构进行表征;同时研究HEPS-Se对人肺癌细胞A549、人前列腺癌细胞DU145的抗增殖作用。结果表明:所制备的HEPS-Se为形貌规则、均一的球体,其元素组成比例为C∶O∶Se∶Pt=32.92∶24.74∶30.46∶11.87;其中Se元素的含量高达481.79μg/g。傅里叶变换红外光谱表明HEPS主要通过—OH、—C=O等基团与纳米硒结合。与HEPS相比,HEPS-Se的粒径显著降低,电位绝对值提高了69.12%。同时,体外抗增殖活性结果表明,HEPS-Se能显著抑制人前列腺癌细胞DU145、人肺癌细胞A549的增值活性,并呈现明显的剂量效应。本研究为有机硒补充剂的开发提供良好的理论依据。
In this study,Hericium erinaceus polysaccharide(HEPS,with a purity of more than 92%)was modified using a redox system consisting of Na_(2)SeO_(3) and ascorbic acid(VC)to obtain selenized HEPS(HEPS-Se).The structural and physiochemical properties of HEPS-Se were investigated by atomic absorption spectrometry(AAS),Fourier transform infrared(FTIR)spectroscopy,X-ray diffractometry(XRD),scanning electron microscopy(SEM),energy-dispersive X-ray(EDX)spectroscopy,X-ray photoelectron spectroscopy(XPS),and nano-zetasizer analysis.The anti-proliferation activity of HEPS-Se on A549 human lung cancer cells and DU145 human prostate cancer cells was evaluated.Results showed that HEPS-Se had a regular spherical shape and four elements C,O,Se and Pt at a ratio of 32.92:24.74:30.46:11.87 were determined in HEPS-Se.Its selenium content was 481.79μg/g.FTIR analysis showed that HEPS could bind to selenium nanoparticles mainly through-OH and-C=O groups.Compared with HEPS,the particle size of HEPS-Se decreased significantly and the absolute potential increased by 69.12%.Meanwhile,HEPS-Se exhibited significantly anti-proliferative effects on DU145 cells and A549 cells in a dose-dependent manner.This study provides a theoretical basis for the development of organic selenium supplements.
作者
古佩娴
尹惠双
胡坤
吴小勇
钟南京
郭娟
黄超
胡勇
陈云
王颖
伍芳芳
GU Peixian;YIN Huishuang;HU Kun;WU Xiaoyong;ZHONG Nanjing;GUO Juan;HUANG Chao;HU Yong;CHEN Yun;WANG Ying;WU Fangfang(School of Food Science,Guangdong Pharmaceutical University,Zhongshan 528458,China;School of Food Science and Engineering,South China University of Technology,Guangzhou 510641,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第22期68-73,共6页
Food Science
基金
广东省教育厅项目(2021KQNCX035,2021ZDZX4001)
广州市科技计划项目(007105356011)
中山市科技计划项目(2020B2068)。
关键词
猴头菇多糖
硒化改性
结构表征
抗增殖活性
Hericium erinaceus polysaccharide
selenization modification
structural characterization
anti-proliferative activity