硒化猴头菇多糖的制备、结构表征及抗增殖活性

Preparation,Structural Characterization and Anti-proliferation Activity of Selenized Hericium erinaceus Polysaccharide

以猴头菇纯化多糖(Hericium erinaceus polysaccharide,HEPS)(总糖含量>92%)为研究对象,在含有VC和亚硒酸钠的氧化还原体系中,制备得到猴头菇多糖纳米硒复合物(HEPS-Se)。通过原子吸收分光光谱仪、傅里叶变换红外光谱仪、X射线衍射仪、扫描电子显微镜、EDX元素分析仪、X射线光电子能谱、纳米粒度仪等对其结构进行表征;同时研究HEPS-Se对人肺癌细胞A549、人前列腺癌细胞DU145的抗增殖作用。结果表明:所制备的HEPS-Se为形貌规则、均一的球体,其元素组成比例为C∶O∶Se∶Pt=32.92∶24.74∶30.46∶11.87;其中Se元素的含量高达481.79μg/g。傅里叶变换红外光谱表明HEPS主要通过—OH、—C=O等基团与纳米硒结合。与HEPS相比,HEPS-Se的粒径显著降低,电位绝对值提高了69.12%。同时,体外抗增殖活性结果表明,HEPS-Se能显著抑制人前列腺癌细胞DU145、人肺癌细胞A549的增值活性,并呈现明显的剂量效应。本研究为有机硒补充剂的开发提供良好的理论依据。

In this study,Hericium erinaceus polysaccharide(HEPS,with a purity of more than 92%)was modified using a redox system consisting of Na_(2)SeO_(3) and ascorbic acid(VC)to obtain selenized HEPS(HEPS-Se).The structural and physiochemical properties of HEPS-Se were investigated by atomic absorption spectrometry(AAS),Fourier transform infrared(FTIR)spectroscopy,X-ray diffractometry(XRD),scanning electron microscopy(SEM),energy-dispersive X-ray(EDX)spectroscopy,X-ray photoelectron spectroscopy(XPS),and nano-zetasizer analysis.The anti-proliferation activity of HEPS-Se on A549 human lung cancer cells and DU145 human prostate cancer cells was evaluated.Results showed that HEPS-Se had a regular spherical shape and four elements C,O,Se and Pt at a ratio of 32.92:24.74:30.46:11.87 were determined in HEPS-Se.Its selenium content was 481.79μg/g.FTIR analysis showed that HEPS could bind to selenium nanoparticles mainly through-OH and-C=O groups.Compared with HEPS,the particle size of HEPS-Se decreased significantly and the absolute potential increased by 69.12%.Meanwhile,HEPS-Se exhibited significantly anti-proliferative effects on DU145 cells and A549 cells in a dose-dependent manner.This study provides a theoretical basis for the development of organic selenium supplements.