基于RBL-2H3模型分析过敏反应效应细胞激活的差异基因

Rat Basophilic Leukemia-2H3 Cells to Analyze the Differential Genes Involved in the Activation of Effector Cells of Anaphylaxis

构建IgE介导的大鼠嗜碱性粒细胞(rat basophilic leukemia,RBL)-2H3作为过敏反应细胞模型,并利用转录组测序分析比较RBL-2H3细胞激活前后的转录组基因差异,富集相关信号通路。IgE介导的RBL-2H3细胞激活后,过敏介质和细胞因子分泌显著提高;转录组测序得到RBL-2H3细胞激活前后的232个差异表达基因,其中有127个(54.74%)可进行基因功能注释;RBL-2H3细胞激活主要影响肿瘤坏死因子、丝裂原活化蛋白激酶、Janus激酶-信号转导与转录激活子、Toll样受体等信号通路,涉及的转录因子有MAP3K8、Nfkbia、Junb、Jun、Fos等。本研究建立了IgE介导的过敏反应体外细胞模型,并且通过对效应细胞转录组信息的全面分析和潜在靶点及信号通路的富集为过敏性疾病的防控治疗提供参考。

IgE-mediated rat basophilic leukemia(RBL)-2H3 cells were used to construct a cell model of anaphylaxis.RNA sequencing was used to analyze differentially expressed genes between unactivated and activated RBL-2H3 cells,and the related signaling pathways were enriched.The activation of IgE-mediated RBL-2H3 cells significantly increased the secretion of allergenic mediators and cytokines.Totally 232 differentially expressed genes were obtained by RNA sequencing,of which 127(54.74%)could be annotated for gene function.Meanwhile,the activation of RBL-2H3 could affect the tumor necrosis factor,mitogen-activated protein kinase,janus kinase-signal transducer and activator of transcription,and Toll-like receptor signaling pathways.The transcription factors involved in these signaling pathways included MAP3K8,Nfkbia,Junb,Jun and Fos.In conclusion,the findings of this study may provide a theoretical foundation for the prevention and treatment of allergic diseases.