一种羊乳粉的微滴式数字PCR定量分析方法

A Microdroplet Digital PCR Assay for Quantitative Analysis of Goat Milk Powder

基于市场上对乳品溯源技术的迫切需求,以微滴式数字聚合酶链式反应技术对羊乳粉中的乳源进行准确判别。基因检测技术对判别乳源来源方面克服了成分复杂、检测时间长等的缺点,更加精准地针对本源进行检测,减少了误判的可能性。本实验以羊乳粉质量、DNA质量浓度及其拷贝数为实验指标,建立了以羊乳粉拷贝数与质量的关系式,准确地判断出羊乳粉的质量,其公式为M=(C-4.75)/3.56,其中,M代表羊乳粉的质量(mg),C代表每微升的拷贝数(copies/μL),该方法的建立对现有市场上的羊乳粉定量检测提供了新的方法和思路。

Driven by the urgent market demand for milk traceability technologies,a microdroplet digital polymerase chain reaction(PCR)assay was developed to accurately identify the milk source in goat milk powder.This method overcame the shortcomings of complex ingredients and long time required for identifying the source of milk,and could detect it more accurately and reduce the possibility of misjudgment.In this study,a model equation for describing the relationship between the number of DNA copies perμL from goat milk powder(C)and its mass(M,mg)was proposed as follows:M=(C−4.75)/3.56.