牡丹皮润制过程的现代技术表征

Modern technical characterization of moistening process of Moutan Cortex

目的:表征牡丹皮润制过程中水分、质构特性变化,探讨牡丹皮润制传统经验的科学内涵。方法:采用吸水动力学曲线评价牡丹皮润制过程中吸水动态变化,采用低场核磁共振技术分析牡丹皮润制过程中水的相态及分布状态变化,采用质构仪测定牡丹皮润制过程中质构特性变化,采用HPLC测定牡丹皮润制过程中有效成分含量变化。结果:吸水率随着润制温度的升高而增大,随着药材档次的增大而减小,建立不同档次牡丹皮10~30℃的吸水动力模型,可预测不同温度不同润制时间的吸水率;润制过程中束缚水和自由水比例不断发生变化,大档牡丹皮5 h内束缚水比例由92.8%减少至14.3%,自由水比例由7.2%增加至85.7%,7 h后药材内全部转化为自由水;低场核磁共振成像表明润制5 h水分已均匀渗入牡丹皮内部,此时内部已无干心;质构特性分析发现,硬度和压缩功随着润制时间的增加不断减小,5 h内硬度由3 612 g减少至444 g,压缩功由208 mJ减少至23 mJ,此时药材软硬适中,5 h后质构特性趋于稳定;随着润制时间的延长,丹皮酚和芍药苷含量逐渐下降,说明润制时间越长,药用物质流失越多。结论:吸水动力学结合低场核磁共振和质构仪等现代技术能够量化表征牡丹皮润制过程,可为牡丹皮润制终点的判断与工艺优化提供数据支撑。

Objective:The changes of moisture and texture characteristics in the moistening process of Moutan Cortex were quantitatively characterized,so as to explore the scientific connotation of the traditional experience of Moutan Cortex moistening.Methods:The dynamic change of water absorption during the moistening process of Moutan Cortex was evaluated by the water absorption kinetics curve.Low-field nuclear magnetic resonance technology was used to analyze the changes of water phase and distribution state during the moistening process of Moutan Cortex.The changes of texture characteristics of Moutan Cortex during moisturizing were measured by texture analyzer.HPLC was used to determine the content changes of active ingredients in the moisturizing process of Moutan Cortex.Results:The water absorption rate was increased with the increment of the moistening temperature,and was decreased with the increasing grade of the medicinal material.The water absorption kinetic model of different grades of Moutan Cortex at 10~30℃was established to predict the water absorption rate of different moisturizing time at different temperatures.During the moistening process,the proportion of bound water and free water changed continuously,within 5 h,the proportion of bound water in high-grade Moutan Cortex was decreased from 92.8%to14.3%,and the proportion of free water was increased from 7.2%to 85.7%,after 7 h,all was transformed into free water.Low field nuclear magnetic resonance imaging showed that water had evenly infiltrated inside the Moutan Cortex after moistening for 5 h and there was no dry core inside at this time.The analysis of texture characteristics showed that the hardness and compression work were decreased with the increase of moistening time,the hardness was decreased from 3612 g to 444 g and the compression work were decreased from 208 m J to 23m J within 5 h,at which time the hardness of the medicinal material was moderate,and the texture characteristics tended to be stable after 5h.The contents of paeonol and paeoniflorin were decreased gradually with the extension of moistening time,indicating that the longer moistening time,the more loss of medicinal substances.Conclusion:Water absorption kinetics combined with modern technologies such as low-field nuclear magnetic resonance and texture analyzer can quantitatively characterize the moistening process of Moutan Cortex,which can provide data support for the judgment of the end point of Moutan Cortex moistening and process optimization.