E3泛素连接酶FBXW7在食管鳞状细胞癌中的表达及对其生物学行为的影响

Expression of E3 ubiquitin ligase FBXW7 in esophageal squamous cell carcinoma and its effect on biological behavior of esophageal squamous cell carcinoma

目的探讨E3泛素连接酶F框/WD-40域蛋白7(FBXW7)在食管鳞状细胞癌(ESCC)中的表达及对其生物学行为的影响。方法通过免疫组织化学的方法检测E3泛素连接酶FBXW7在171例ESCC组织及53例癌旁组织中的表达,探究FBXW7的表达对ESCC患者生存预后及临床病理特征之间的关系。构建过表达FBXW7组ESCC细胞和对照组ESCC细胞,采用CCK-8实验、平板克隆形成实验、Transwell细胞迁移和侵袭实验、细胞划痕实验检测FBXW7对ESCC细胞增殖、迁移和侵袭能力的影响。结果FBXW7在癌旁组织中表达高于ESCC组织(P=0.002),FBXW7低表达的ESCC分化程度更低(P=0.011),FBXW7高表达的ESCC患者TNM分期更早(P=0.032),且FBXW7高表达患者生存期长于FBXW7低表达患者(P=0.030);CCK-8实验结果显示过表达FBXW7组细胞吸光度低于对照组(P<0.001),平板克隆形成实验结果显示过表达FBXW7组细胞集落数低于对照组(P<0.001),细胞划痕实验结果显示过表达FBXW7组细胞迁移率低于对照组(P<0.001),Transwell细胞迁移和侵袭实验结果显示过表达FBXW7组细胞迁移和侵袭数目均低于对照组(P<0.001)。结论FBXW7在ESCC中低表达,并且FBXW7低表达提示患者不良预后,过表达FBXW7可以抑制ESCC细胞的增殖和迁移侵袭能力。

Objective To investigate the expression of E3 ubiquitin ligase F-box and WD-40 domain protein 7(FBXW7)in esophageal squamous cell carcinoma(ESCC)and its effect on its biological behavior.Methods The expression of E3 ubiquitin ligase FBXW7 in 171 cases of ESCC and 53 cases of paracancer tissues was detected by immunohistochemistry.The relationship between FBXW7 expression and survival prognosis and clinicopathological features of patients with ESCC was explored.The ESCC cells of the overexpressed FBXW7 group and the ESCC cells of the control group were constructed,and the effects of FBXW7 on the proliferation,migration and invasion of ESCC cells were detected by CCK-8 assay,plate clone formation assay,Transwell cell migration and invasion assay and cell scratches assay.Results The expression of FBXW7 in paracancer tissues was higher than that in ESCC tissues(P=0.002).ESCC with low FBXW7 expression had lower differentiation degree(P=0.011)and TNM stage was earlier in ESCC patients with high FBXW7 expression(P=0.032),and the survival time of patients with high FBXW7 expression was longer than that of patients with low FBXW7 expression(P=0.030).CCK-8 assay results showed that the absorbance of cells in overexpressed FBXW7 group was lower than that in control group(P<0.001),and plate clone formation assay showed that the colony number of overexpressed FBXW7 group was lower than that of the control group(P<0.001).Cell scratches assay results showed that the cell migration rate of the overexpressed FBXW7 group was lower than that of the control group(P<0.001),Transwell cell migration and invasion assay showed that the number of cell migration and invasion in the overexpressed FBXW7 group was lower than that in the control group(P<0.001).Conclusion The expression of FBXW7 is low in ESCC and low expression of FBXW7 suggests poor prognosis.Overexpression of FBXW7 can inhibit the proliferation,migration and invasion of ESCC cells.