体外细胞周期拉伸力加载系统的研制及工作性能测试

Development and performance test of cell cyclic tensile force loading system in vitro

目的由于拉伸应力是一种重要的机械应力刺激形式,为了研究拉伸应力对骨组织形成和骨细胞功能的影响,构建了一套体外细胞周期拉伸力加载系统。方法首先,自主设计并构建加载系统,通过真空吸附柔性硅胶膜的方式,系统能够产生可控大小和频率的机械牵张应力;其次,分析不同减压阀设定值下膜应变值与梯度拉伸应力间的关系,并对膜应变的线性进行测试;最后,分析硅胶膜上感兴趣区内同一加载应力下不同方向以及不同循环加载次数后的膜应变值,并对膜应变的各向同性和膜的顺应性进行测试。结果自主设计构建的机械牵张应力加载装置能够在硅胶膜上感兴趣区内施加线性、各向同性且可重复的拉伸应变。结论通过对加载系统的性能测试,证明了使用本装置施加双轴牵张应力刺激的可行性和可靠性,为日后研究拉伸应力对其他各种细胞的生物学效应提供了良好的硬件基础。

Objective Since tensile stress is an important form of mechanical stress stimulation,in order to study the effects of tensile stress on bone tissue formation and osteocyte function,an in vitro cell cyclic tensile force loading system is constructed.Methods Firstly,a closed-loop feedback loading system of in vitro cell cyclic tensile force was designed and constructed independently.By means of vacuum adsorption of flexible silicone membrane,the system could generate mechanical tensile stress of controllable magnitude and frequency.Secondly,by analyzing the relationship between membrane strain and gradient tensile stress under different pressure reducing valve settings,the linearity of membrane strain was tested.Finally,by analyzing the membrane strain values in various directions and after different loading cycles under the same loading stress in the region of interest on the silicone membrane,the isotropy of membrane strain and membrane compliance were measured.Results The self-designed and constructed mechanical tensile stress loading system could apply linear,isotropic and repeatable tensile strains in the region of interest on the silicone membrane.Conclusion The performance test of the loading system proves the feasibility and reliability of utilizing this device to apply biaxial tensile stress stimuli,which provides a favorable hardware basis for future study of the biological effects of tensile stress on other cells.