超声辅助低共熔溶剂提取鳞杯伞多糖工艺优化及结构和流变学特性

Ultrasound Assisted Extraction of Polysaccharides from Clitocybe squamulosa with Deep Eutectic Solvents:Optimization,Structure and Rheological Properties

采用单因素和响应面法优化超声辅助低共熔溶剂提取鳞杯伞多糖(D-CSFP)的工艺,测定D-CSFP的得率和多糖含量,鉴定其结构,研究其流变学特性。结果表明:D-CSFP的最佳提取工艺为提取时间40 min、提取温度75℃、料液比1∶24(g∶mL),在此条件下提取率、得率、多糖含量分别为(5.31±0.09)%、(6.52±0.29)%、(81.42±0.59)%。D-CSFP的重均分子量(M_(w))和数均分子量(M_(n))分别为35312、24494,单糖组成为葡萄糖、甘露糖、半乳糖、盐酸氨基葡萄糖、木糖,其摩尔比为6.53∶2.04∶1.23∶0.1∶0.09。D-CSFP呈现出良好的流变学特性,可作为一种新型水凝胶体应用于食品、医药和化妆品等领域。研究结果为食用菌多糖的提取提供新思路,也为D-CSFP产品开发提供参考。

Deep eutectic solvents were used in ultrasound assisted extraction of polysaccharides from Clitocybe squamulosa(D-CSFP).Single factor experiments and response surface design were carried out to optimize the extraction process,and the derived D-CSFP was measured for extraction rate and polysaccharide content,analyzed for structure and studied for its rheological properties.The results showed that the optimal extraction conditions for D-CSFP were follows:extraction time 40 min,extraction temperature 75℃,and solid-liquid ratio 1∶24(g∶mL).Under these conditions,the extraction rate,yield and polysaccharide content were(5.31±0.09)%,(6.52±0.29)%,and(81.42±0.59)%,respectively.The weight average molecular weight(M_(w))and number average molecular weight(M_(n))of D-CSFP were 35312 and 24494,respectively.D-CSFP was composed of glucose,mannose,galactose,glucosamine hydrochloride and xylose in the molar ratio of 6.53∶2.04:1.23∶0.1∶0.09.D-CSFP showed good rheological properties,and thus can be used as a new hydrocolloid in the food,medicine and cosmetics industries.This study provided a reference for green extraction of edible fungi polysaccharides,and a basis for the development of D-CSFP products.