甘油致不同性别SD大鼠急性肾脏损伤模型的评价

Evaluation of a glycerol-induced acute kidney injury model in SD rats

目的:探讨甘油经肌肉注射对不同性别大鼠急性肾脏损伤的影响。方法:将24只健康成年SPF级SD大鼠(雌雄各半)按性别随机分为4组,雄性对照组、雄性试验组、雌性对照组与雌性试验组,每组6只。试验前禁水24 h,按照10 mL/kg的剂量分别给予大鼠两侧后肢肌肉注射生理盐水或50%甘油生理盐水,用代谢笼采集24 h尿液;并在注射48 h后麻醉通过腹主动脉采集血液,手术摘取肾、肝、脾等脏器,检查血清生化、血常规、尿常规及血凝等指标,观察肾、肝、脾等脏器的病理学变化。结果:与对照组比较,试验组大鼠血液中白蛋白(ALB)、红细胞(RBC)均出现明显降低(P<0.05),而肌酐、尿素氮、谷丙转氨酶、血小板均明显升高(P<0.05);雌性试验组大鼠的总蛋白及尿比重均明显降低,而门冬氨酸氨基转移酶出现明显升高(P<0.05);病理结果显示试验组动物肾小管扩张,可见透明管型与蛋白管型,肝血窦发生扩张,其中肾损伤最明显。结论:肌肉注射甘油不仅造成肾脏损伤,而且还造成轻微的肝脏损伤。在甘油诱导的急性肾损伤模型中,雄性大鼠的肾损伤程度低于雌性大鼠。

OBJECTIVE:To explore effects of intramuscular injection of glycerol on kidneys,the main blood storage organ,of rats.METHODS:Twenty-four healthy adult SPF SD rats(half male and half female)were randomly divided into four groups of six rats per group:control and test groups for each sex.After 24 hours of water deprivation,rats were given either normal saline or 50%glycerol normal saline at the dose of 10 mL/kg.After another 24 hours,anesthesia was performed and blood was collected through the abdominal aorta.The kidney,liver and spleen were removed surgically and examined for pathological damage.Urine samples were collected from their metabolic housing and examined for serum biochemistry,blood routine,urine routine and hemagglutination.RESULTS:The results showed that albumin(ALB)and red blood cell(RBC)in the glycerol male and female groups were reduced significantly(P<0.05),while creatinine(Crea),urea nitrogen(Urea),alanine aminotransferase(ALT)and platelet(PLT)were increased significantly(P<0.05).Total protein(TP)and specific gravity of urine(SG)of female animals in the glycerol group were reduced significantly,while aspartate aminotransferase(AST)was increased significantly(P<0.05).Pathological results showed that the renal tubules in the experimental groups were dilated,including clear tube type and protein tube type.The hepatic sinuses were dilated and the renal injury was the most obvious.CONCLUSION:Intramuscular injection of glycerol not only caused kidney damage,but also caused slight liver damage.In this glycerol induced acute renal injury model,the degree of renal injury in male rats was lower than that in female rats.