摘要
目的:研究慢性鼻窦炎伴鼻息肉(chronic sinusitis with nasal polyps,CRSwNP)患者外周血中髓源性抑制细胞(myeloid-derived suppressor cells,MDSCs)及其亚群比例和功能的变化,探讨MDSCs对CRSwNP疾病进展的影响。方法:采用流式细胞术检测CRSwNP组(31例)及对照组(36例)外周血中HLA-DR-CD11b+CD33+MDSCs,HLA-DR-CD11b+CD33+CD14-CD15+粒细胞性MDSCs(PMN-MDSCs)和HLA-DR-CD11b+CD33+CD14+CD15-单核细胞性MDSCs(M-MDSCs)的比例和功能分子精氨酸酶-1(arginase-1,ARG-1)的表达情况,同时采用ELISA检测血浆中IL-6的浓度。结果:CRSwNP患者外周血总MDSCs比例明显高于对照组,差异有统计学意义(P<0.01);与对照组比较,CRSwNP组PMN-MDSCs比例明显升高(P<0.05),而M-MDSCs比例下降(P<0.05)。CRSwNP组总MDSCs中ARG-1的平均荧光强度升高(P<0.05),血浆IL-6浓度增加(P<0.05)。结论:CRSwNP患者中总MDSCs的表达升高且以PMN-MDSCs为主,其功能分子ARG-1表达增强,提示MDSCs可能参与了慢性鼻窦炎的发病过程。
Objective:To investigate the changes in the ratio and function of myeloid-derived suppressor cells(MDSCs)and its subgroups in patients with chronic sinusitis with nasal polyp(CRSwNP),and explore the influence of MDSCs on the occurrence and development of CRSwNP.Methods:Flow cytometry(FCM)was used to detect the ratio of HLA-DR-CD11b+CD33+MDSCs,HLA-DR-CD11b+CD33+CD14-CD15+PMN-MDSCs and HLA-DR-CD11b+CD33+CD14+CD15-M-MDSC,and the expression of the functional molecule arginase-1(ARG-1)in peripheral blood in the CRSwNP group(31 cases)and control group(36 cases).At the same time,the concentration of IL-6 in plasma was detected by enzyme-linked immunosorbent assay.Results:The proportion of total MDSCs in the peripheral blood of CRSwNP patients was significantly higher than that of the control group(P<0.01).Among them,the proportion of PMN-MDSCs in the CRSwNP group was significantly increased(P<0.05);while the proportion of M-MDSCs decreased(P<0.05).The average fluorescence intensity of ARG-1 in the total MDSCs in the CRSwNP group increased(P<0.05),and the plasma IL-6 concentration also increased(P<0.05).Conclusion:The expression of total MDSCs in CRSwNP patients is increased and PMN-MDSCs are the dominant ones,and the expression of ARG-1 is also increased.It suggests that MDSCs may be involved in the occurrence and development of chronic sinusitis.
作者
吴会会
杨珺
曾炫富
王娅
王胜军
马永明
WU Huihui;YANG Jun;ZENG Xuanfu;WANG Ya;WANG Shengjun;MA Yongming(School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013;Department of Otorhinolaryngology Head and Neck Surgery,People′s Hospital Affiliated to Jiangsu University,Zhenjiang Jiangsu 212002;Department of Laboratory Medicine,People′s Hospital Affiliated to Jiangsu University,Zhenjiang Jiangsu 212002,China)
出处
《江苏大学学报(医学版)》
CAS
2022年第6期504-508,共5页
Journal of Jiangsu University:Medicine Edition
基金
江苏省卫生健康委科研项目(K2019019)
江苏省医学重点人才项目(ZDRCB2016018)
江苏大学附属人民医院院级课题(Y2020007)。
关键词
慢性鼻窦炎
鼻息肉
髓源性抑制细胞及其亚型
免疫抑制
精氨酸酶-1
白介素-6
chronic sinusitis
nasal polyps
myeloid-derived suppressor cells and their subgroups
immunosuppression
arginase-1
interleukin-6