红景天苷对Lewis荷瘤小鼠调节性T细胞的抑制作用

Salidroside inhibits Treg function in Lewis lung carcinoma mice

为探讨红景天苷(salidroside, SAL)的抗肿瘤免疫功能,将3LL肺癌荷瘤小鼠随机分为PBS组、SAL治疗组(6 mg/kg)和紫杉醇(paclitaxel, PTX)阳性对照组(2 mg/kg),分别治疗21 d后绘制肿瘤体积生长曲线;采用FACS检测肿瘤浸润组织Foxp3^(+)Treg的比例,检测Treg表面分子CD69及核内分子Ki67的表达,检测联合培养条件下SAL对Treg及CD4^(+)CD25^(-)T细胞的影响。结果显示,SAL组小鼠肿瘤生长受到显著抑制(P<0.05),Treg表面分子CD69和核内分子Ki67的表达显著下调(P<0.05);与PBS组比较,SAL组小鼠Treg和效应T细胞联合培养的效应T细胞的数量显著增加(P<0.05)。该研究提示,SAL对Treg的抑制功能可能产生影响,从而抑制肿瘤生长。

To explore the anti-tumor function of salidroside(SAL),this study used 3 LL lung cancer-bearing mice randomly divided into three groups:a PBS group,a SAL group(6 mg/kg),and a paclitaxel(PTX,2 mg/kg)group included as the positive control.Tumor growth curves were generated 21 days after treatments.In addition,FACS analysis was used to evaluate the efficacy of SAL treatment on Foxp3^(+)Treg composition in the tumor-infiltrating tissue,the expression levels of CD69 and Ki67 in Treg,and the variations of Treg and CD4^(+)CD25^(-)T cells in a coculture system.The results showed that tumor growth was significantly inhibited in the SAL group(P<0.05),and the expressions of CD69 and Ki67 were significantly down-regulated(P<0.05).Compared to the PBS group,the proportion of effector T cells in the coculture system of Treg and effector T cells increased significantly upon SAL treatment(P<0.05).In summary,this study suggests that SAL may exert anti-tumor activity by inhibiting Treg function.