山莓果实不同极性萃取物降血糖作用研究

Hypoglycemic Effect of Different Solvent Extracts fromRubus Corchorifolius Fruits

目的:研究山莓果实不同极性萃取部位的降血糖作用,筛选出最佳活性部位,为其降血糖活性成分研究及综合开发利用提供实验数据和科学依据。方法:采用超声提取法得到山莓果实乙醇粗提物,后用不同极性的溶剂进行萃取,得到4个不同极性萃取部位(石油醚、乙酸乙酯、正丁醇和水层);采用α-葡萄糖苷酶及α-淀粉酶抑制模型评价不同极性萃取物降血糖活性。结果:在降血糖作用中,正丁醇层对α-葡萄糖苷酶活性、猪胰腺α-淀粉酶活性抑制效果最好,其IC_(50)分别为(0.186±0.00503)mg/mL、(2.269±0.04876)mg/mL。结论:山莓果实4个萃取部位均具有一定降血糖作用,降血糖作用的最佳极性部位为正丁醇层。

Objective:To study the hypoglycemic effect in four different polar sites of Rubus corchorifolius Fruits,which that provides experimental data and scientific basis for the pharmacological research and the development of future study and utilization in the way of hypoglycemic active components.Methods:The ethanol extracts of Rubus corchorifolius Fruits were gained by using ultrasonic vibration method;Petroleum ether,ethyl acetate,n-butanol and water layer were extracted by organic solvent extraction;The activities ofα-glucosidase and porcine pancreaticα-amylase in Rubus corchorifolius Fruits were detected by enzyme-labeled instrument.Results:The inhibition ofα-glucosidase and porcine pancreaticα-amylase activity were the strongest in n-butanol layer,its IC_(50) were(0.186±0.00503)mg/mL、(2.269±0.04876)mg/mL.Conclusion:N-butanol laye had the best extraction site for hypoglycemic effect.