短梗五加多糖的结构解析及抗氧化活性分析

Structural analysis and hypoglycemic activity analysis of polysaccharides from Acanthopanax sessiliflorus

对短梗五加中的多糖组分进行分离纯化,通过实验解析组分结构,并对其抗氧化活性进行分析。从短梗五加茎中分离纯化短梗五加多糖,通过高效液相色谱法、气相色谱-质谱分析、傅里叶变换红外光谱和紫外-可见光光谱法、^(1)H NMR和^(13)C NMR等对其组成结构特征进行研究和分析,并考察了短梗五加多糖对1,2-二苦基-2-三硝基苯亚肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)的抗氧化能力和铁离子还原能力。实验结果表明,短梗五加多糖由甘露糖、葡萄糖、半乳糖、阿拉伯糖组成,结构主要由吡喃糖环状构成,可以有效清除DPPH、ABTS阳离子自由基,还原铁离子。证明短梗五加多糖具有较好的抗氧化生物活性。

The aim was to isolate and purify the polysaccharide components in Dangsengwujia,analyze the structure of the components through experiments,and analyze their antioxidant bioactivity.The polysaccharides named ASP were isolated and purified from the stems of Acanthopanax sessiliflorus,and their structural characteristics were studied and analyzed by high performance liquid chromatography,gas chromatography-mass spectrometry,Fourier transform infrared,ultraviolet-visible spectroscopy,^(1)H NMR and ^(13)C NMR.The antioxidant activities and iron ion reduction ability of polysaccharide of ASP to 1,2-dipicyl-2-trinitrophenylhydrazine(DPPH),and 2,2-biazo-bis(3-ethyl-benzothiazol-6-sulfonic acid)ammonium salt(ABTS)were evaluated.The experimental results show that the polysaccharide of ASP is composed of mannose,glucose,galactose and arabinose,and its structure is mainly composed of pyranose rings.It can effectively scavenge DPPH,ABTS cation free radicals,reduce iron ions,and show a certain antioxidant capacity.The polysaccharides from ASP have good antioxidant bioactivity.