高通量悬浮芯片检测仪器光学系统的设计与实现

Design and Implementation of Optical System of High Flux Suspension Chip Testing Instrument

基于流式荧光技术,针对高通量悬浮芯片的编码微球解码分析技术,研究了一款流式点阵仪的光学系统。该仪器采用创新性光学系统设计,在激光光斑整形光路中,采用二次整形透镜复用设计,保证了不同波长激发出的荧光信号形态、宽度完全一致。整个光学系统的功能区块采用一体化设计,各模块安装可以一次性完成,使得安装或更换更为便利;光学模块整体集成度高、结构精简,通过光路精确计算和机械加工来确保安装位置的准确性。

Based on the flow fluorescence technology and the coded microspheres decoding analysis technology of high throughput suspension chip,an optical system of flow dot matrix instrument is studied.The instrument adopts an innovative optical system design.In the laser spot shaping optical path,the secondary shaping lens multiplexing design is adopted to ensure that the shape and width of fluorescence signals excited by different wavelengths are completely consistent.The functional blocks of the whole optical system are designed in an integrated way,and the installation of each module can be completed at one time,which is more convenient for installation or replacement;the optical module has high overall integration and simplified structure.The accuracy of installation position is ensured through accurate optical path calculation and mechanical processing.