不同温度下中间球海胆生长预测模型构建

Construction of Growth Prediction Models of Sea Urchin Strongylocentrotus intermedius Exposed to Different Water Temperatures

将壳径2 cm、4 cm和6 cm的中间球海胆Strongylocentrotus intermedius饲养在控温循环水槽中,每组120只,水温控制在10℃、14℃、18℃、22℃、24℃或26℃,每组设3个平行。每两天投喂一次石莼(Ulva sp.),吸取水槽底部粪便,换水1/5,水温波动在0.2℃之内。实验进行5周,每周称量一次海胆体质量,计算平均增重率(WGR),分析不同壳径中间球海胆生长最适温度和极限温度,利用多项式拟合和响应曲面法建立温度影响下中间球海胆生长预测模型。结果显示,在实验温度范围内,中间球海胆增重率随温度的升高而上升;超过最适温度后,海胆增重率下降;当水温超出海胆耐热极限后,中间球海胆体质量减轻直至死亡。温度影响中间球海胆生长预测模型为抛物线状,模型R2值大于0.94,模型构建合理有效。模型计算得出,壳径2 cm、4 cm和6 cm中间球海胆生长最适温度分别为16.1℃、15.4℃和13.6℃,生长极限温度分别为25.1℃、24.5℃和21.0℃。随壳径增大,中间球海胆生长最适温度和极限温度均下降。中间球海胆生长对不同温度的响应均可用二次回归曲线进行拟合,可为中间球海胆工厂化温度调控提供一定的理论依据。

The sea urchin(Strongylocentrotus intermedius)with diameter of 2 cm,4 cm,and 6 cm was reared in a temperature-controlled circulating water tank at a stocking density of 120 at water temperature of 10℃,14℃,18℃,22℃,24℃ and 26℃,with triplication,and fed sea lettuce(Ulva sp.)every two days,siphoning feces from the bottom of the tank,and exchange of 1/5 water for 5weeks to evaluate the influence of temperature on the growth of the sea urchin via weight gain rate(WGR)and to develop growth prediction model of the sea urchin by polynomial fitting and response surface method.The results showed that the sea urchin had increase in WGR with the increasing temperature,with decreasing in WGR beyond the optimum temperature and degrowth(loss WGR)until to death exceeding the heat resistance limit.The sea urchin had a parabolic growth prediction model under different temperature conditions,with R2value of over 0.9369,indicating that the model construction was reasonable and effective.The calculation by the model that the sea urchin had optimum growth temperature of 16.1℃ in the 2 cm diameter individuals,15.4℃ in the 4 cm diameter individuals,and 13.6℃ in the 6 cm diameter individuals.The sea urchin had the growth limit temperature of 25.1℃ in the 2 cm diameter individuals,24.5℃ in the 4 cm diameter individuals,and 21.0℃ in the 6 cm diameter individuals,indicating the optimum and extreme temperature for growth of the sea urchin showed a downward trend with the diameter increasing and that the growth response of the sea urchin to different temperatures can be fitted with quadratic regression curves.The finding provides a theoretical basis for temperature control in the process of the sea urchin industrial aquaculture.