黑龙江省桦南县2020年蜱媒病毒调查

Investigation of tick-borne viruses in Huanan county,Heilongjiang province,China,2020

目的调查黑龙江省佳木斯市桦南县蜱及蜱媒病毒种类。方法于2020年5月在桦南县采集蜱标本,并进行形态学鉴定。利用实时荧光定量PCR方法对采集的蜱标本进行蜱传脑炎病毒(TBEV)、发热伴血小板减少综合征病毒、鄂木斯克出血热病毒、兰加特病毒、波瓦森病毒、阿龙山病毒核酸检测,并使用高通量测序法获得阳性病毒基因组序列。结果共采获890只蜱,包括全沟硬蜱475只(雄蜱210只、雌蜱265只),森林革蜱360只(雄蜱260只、雌蜱100只),嗜群血蜱55只(雄蜱5只、雌蜱50只)。1批(10只蜱混合)标本(JMS20)TBEV核酸阳性,由雌性全沟硬蜱携带。其他病毒核酸全部阴性。系统进化显示,该株蜱传脑炎病毒与远东型TBEV亲缘关系较近。结论佳木斯地区蜱媒样本仍携带蜱传脑炎病毒,研究结果为该地区蜱传病毒病的防控提供了基础数据。

Objective To investigate the species of ticks and tick-borne viruses in Huanan county,Jiamusi city,Heilongjiang province,China.Methods Ticks were collected in Huanan in May 2020 and identified according to morphological keys.Real-time PCR was used to detect the RNA of viruses in the collected ticks,i.e.,tick-borne encephalitis virus(TBEV),severe fever with thrombocytopenia syndrome virus(SFTSV),Omsk hemorrhagic fever virus(OHFV),Langat virus(LGTV),Powassan virus(POWV),and Alongshan virus(ALSV).High-throughput sequencing was used to obtain the genome sequences of detected viruses.Results A total of 890 ticks were collected,including 475 Ixodes persulcatus(210 males and 265 females),360 Dermacentor silvarum(260 males and 100 females),and 55 Haemaphysalis concinna(5 males and 50 females).One batch of tick specimens(n=10;JMS20)was positive for TBEV,which was carried by female I.persulcatus.Other viruses were not detectable.Phylogenetic analysis showed that this TBEV strain was closely related to the Far Eastern subtype of TBEV.Conclusion Ticks in Jiamusi city still carry TBEV,and our results provide basic data for the prevention and control of tick-borne virus diseases in the region.