牛病毒性腹泻病毒检测方法研究进展

Progress on Detection Methods of Bovine Viral Diarrhea Virus

牛病毒性腹泻(BVD)是一种由牛病毒性腹泻病毒(BVDV)引起的接触性传染病,可感染不同年龄阶段的牛,其中幼龄牛易感性最高,常导致患病动物出现呼吸系统、消化系统和生殖系统临床症状,并可引起免疫系统抑制。该病在世界范围内流行,是危害全球养牛业的主要疫病之一,我国也不例外。因此,建立快速、准确的检测方法对该病的防控尤为重要。论文从病原学诊断、血清学方法和分子生物学方法对国内外BVDV检测方法进行综述,并对各种方法的优缺点及适用性进行总结归纳,以期为BVD的防控和净化提供参考。

Bovine viral diarrhea(BVD)is a contagious infectious disease caused by bovine viral diarrhea virus(BVDV).It can infect cattle at different ages,of which young cattle is the most susceptible.It often leads to clinical symptoms of respiratory system,digestive system and reproductive system in diseased animals,and can cause suppression of immune system.The disease is prevalent all over the world.It is one of the main diseases endangering the global cattle industry,and China is no exception.Therefore,the establishment of rapid and accurate detection methods is particularly important for the prevention and control of the disease.This paper summarized the detection methods of BVDV at home and abroad from three aspects:pathogenic diagnosis,serological methods and molecular biological methods,and summarized the advantages,disadvantages and applicability of each method,in order to provide a theoretical basis for the prevention,control and purification of BVD.