摘要
为建立蕲蛇及其混伪品的生物信息学鉴别方法,采用随机扩增DNA多态性技术(RAPD)扩增蕲蛇线粒体细胞色素b(Cyt b),将扩增产物应用高效毛细管电泳技术(HPCE)和紫外线检测技术进行检测,对影响RAPD和HPCE的因素进行优化,建立蕲蛇的HPCE-RAPD指纹图谱,并对所得指纹图谱进行相似性分析发现,蕲蛇正品及伪品的指纹图谱相似性存在显著性差异。故本方法不仅适用于蕲蛇及其伪品的快速鉴别,再进一步研究的基础上也可广泛应用于其他易混中药的快速鉴别。
In order to establish a bioinformatics identification method for Agkistrodon acutus and its adulterants,the mitochondrial cytochrome b(Cyt b)of Agkistrodon acutus was amplified by randomly amplified DNA polymorphism(RAPD).The amplified products were detected by high performance capillary electrophoresis(HPCE)and ultraviolet detection technology.The factors affecting RAPD and HPCE were optimized,the HPCE-RAPD fingerprint of Agkistrodon acutus was established.Meanwhile the similarity of the obtained fingerprint was analyzed.It was found that there were significant differences in the similarity of the fingerprints between the authentic and counterfeit Agkistrodon acutus.Therefore,this method is not only suitable for the rapid identification of Agkistrodon acutus and its counterfeits,but also can be widely used for the rapid identification of other easily mixed Chinese medicines on the basis of further research.
作者
刘桐辉
LIU Tonghui(School of Pharmaceutical and Environmental Technology,Jilin Industrial Vocational and Technical College,Jilin 132013,China)
出处
《技术与教育》
2022年第2期9-13,26,共6页
Technique & Education
基金
吉林工业职业技术学院2020年度院级课题“中药易混品种DNA指纹技术的研究与应用”(课题编号:20ky07)
吉林省科技厅吉林省科技发展计划2021年度项目“系列中药饮片基因快速检测试剂的产业化研究”(课题编号:20210401109YY)的研究成果之一。
