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河北邢台及周边地区猪圆环病毒2型分子流行病学调查 被引量:1

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摘要 为初步了解当前河北省猪圆环病毒2型流行情况,于2021年10月至2022年6月从河北邢台市及周边地区79个猪场共收集205份病猪组织样品,采用实时荧光定量PCR方法检测病料样品中PCV2核酸阳性率;同时采用PCR法扩增部分PCV2阳性样品ORF2基因序列,分析毒株基因型和基因变异情况。结果发现,送检样品中,PCV2检测场阳性率和样品阳性率分别为51.90%(41/79)和32.68%(67/205),其中不同地区场阳性率和样品阳性率分别为22.22%~63.64%和20.83%~43.33%;此外,不同类型猪场和不同临床症状猪群来源样品PCV2检出率存在较大差异。10份PCV2阳性样品ORF2基因序列成功扩增(均为705 nt),其中6份样品ORF2基因核苷酸序列与已知PCV2d毒株同源性最高,4份样品与PCV2b毒株同源性最高,提示PCV2b和PCV2d可能为河北省主要流行的基因型。
出处 《养猪》 2022年第6期113-115,共3页 Swine Production
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