闽楠PLR基因家族鉴定及响应激素的表达分析

Identification of PLR gene family and expression of responsive hormones in Phoebe bournei

【目的】解析闽楠Phoebe bournei木脂素合成途径关键酶基因Pinoresinol-lariciresinol reductase (PLR)特征及不同激素处理下的表达模式,探索闽楠PLR基因家族的生物学功能。【方法】利用生物信息学方法鉴定闽楠基因组中的PLR基因家族成员,分析基因结构、保守基序、染色体定位、系统进化、启动子顺式作用元件和激素响应。【结果】从闽楠全基因组内共鉴定出8个闽楠PLR (PbPLR)成员,不均匀地分布于第1、2、5和10号染色体。系统进化分析表明:PbPLR功能可能与底物立体选择性有关。启动子元件分析发现:PbPLRs启动子区域存在脱落酸、水杨酸和茉莉酸甲酯等激素响应元件;基因表达分析表明:PbPLRs基因的表达具有组织特异性和激素响应特征,其中PbPLRs基因在根中表达量最高,其次茎,叶中表达量较低;3种激素处理时,PbPLR2表达诱导最强。【结论】从闽楠基因组中鉴定出的8个PbPLRs基因,其基因特征和不同成员可能具有不同催化酶立体选择性,成员表达模式多样化,可能受多种激素诱导,具有组织特异性。图6表2参25。

[Objective] Pinoresinol-laricisinol reductase(PLR) is a key enzyme gene involved in lignan biosynthesis. This study aims to clear the characteristics of PLR gene family in Phoebe bournei and the expression patterns under different hormone treatments, so as provide basis for further exploring the biological function of PLR gene family in P. bournei. [Method] The members of PLR gene family in P. bournei genome were identified by bioinformatics methods, and the gene structure, conserved motifs, chromosome location,phylogeny, cis acting elements of promoter and hormone response were analyzed. [Result] A total of 8 PLR(PbPLR) members were identified from the genome of P. bournei, which were unevenly distributed on chromosomes 1, 2, 5 and 10. Phylogenetic analysis indicated that PbPLR function may be related to substrate stereoselectivity. Promoter element analysis showed that there were hormone response elements such as bascisic acid(ABA), salicylic acid(SA), and methyl jasmonate(MeJA) in the promoter region of PbPLRs. Gene expression analysis showed that the expression of PbPLRs gene had the characteristics of tissue specificity and hormone response. The expression of PbPLRs gene was the highest in roots, followed by stems and leaves. The expression of PbPLR2 was the strongest when treated with 3 hormones. [Conclusion] The 8 PbPLRs gene identified from the genome of P. bournei have different gene characteristics, and different members may have different stereoselectivity of catalytic enzymes. The expression patterns of members are diverse, which may be induced by various hormones and have tissue specificity. [Ch, 6 fig. 2 tab. 25 ref.]