红景天苷对DCM大鼠血清中HIF-1α、ET-1、PⅠP、醛固酮的影响

Effect of Salidroside on Serum HIF-1α, ET-1, PⅠP and Aldosterone in DCM Rats

目的 观察红景天苷对糖尿病心肌病(DCM)大鼠血清中缺氧诱导因子1α(HIF-1α)、内皮素-1(ET-1)、Ⅰ型胶原(PⅠP)及醛固酮的影响,并探讨可能的心脏保护机制。方法 取大鼠40只,用链尿佐菌素(STZ)造DCM大鼠模型,成模后随机分为对照组、红景天苷中剂量组(50 mg/kg)、红景天苷高剂量组(100 mg/kg)、曲美他嗪组(2 mg/kg),连续灌胃给药12周,末次给药后禁食12 h,麻醉后腹主动脉取血标本,检测血清中HIF-1α、ET-1、PⅠP、醛固酮含量。结果 对照组血清中HIF-1α、PⅠP含量显著高于红景天苷中、高剂量组及曲美他嗪组(P<0.05);对照组血清中醛固酮、ET-1测值显著高于红景天苷中、高剂量组(P<0.05);且红景天苷高剂量组在降低血清醛固酮含量方面明显优于曲美他嗪组(P<0.05)。结论 红景天苷对DCM大鼠的治疗作用明显,其作用机制可能是通过抑制肾素-血管紧张素-醛固酮系统(RAAS系统)、抑制心肌纤维化、改善心肌细胞的氧供及血管内皮功能等来实现的。

Objective To observe the effects of salidroside on serum HIF-1α, ET-1, PⅠP and aldosterone in DCM rats, and to explore the possible cardiac protective mechanism. Methods Forty rats were used to make DCM model with STZ. After modeling, they were randomly divided into control group, salidroside medium-dose group(50 mg/Kg), salidroside high-dose group(100 mg/kg) and trimethazine group(2 mg/kg). The rats were given continuous intragastric administration for 12 weeks and fasted for 12 hours after the last administration. The contents of HIF-1α, ET-1, P ⅠP and aldosterone in serum were detected. Results The serum contents of HIF-1α and PⅠP in control group were significantly higher than those in salidroside medium-high dose group and trimetazidine group(P<0.05). The serum aldosterone and ET-1 values in control group were significantly higher than those in medium-dose and high-dose salidroside groups(P<0.05). And salidroside high dose group was significantly better than trimetazidine group in reducing serum aldosterone content(P<0.05). Conclusion Salidroside has obvious therapeutic effect on DCM rats, and its mechanism may be realized by inhibiting RAAS system, inhibiting myocardial fibrosis, improving myocardial oxygen supply and vascular endothelial function.