壳聚糖-马来酸/丙烯酰胺双网络型超分子水凝胶制备及应用

Preparation and Application of Chitosan-Maleic Acid/Acrylamide DoubleNetwork Supramolecular Hydrogel

利用干法研磨制备羧甲基-β-环糊精(CM-β-CD),将单宁酸(TA)嵌入形成包合物;通过自由基反应在壳聚糖(CS)中原位共聚马来酸酐(MAH)及丙烯酰胺(AM),再将包合物负载壳聚糖上,壳聚糖与P(MAH-r-AM)间离子配位形成TA-CM-β-CD/CS/MAH-AM双网络超分子水凝胶。采用红外光谱、扫描电镜、电子万能材料实验仪及生物实验对其进行性能测试。结果表明,当TA与CM-β-CD的质量比为1∶1(50 mg)时,TA-CM-β-CD/CS/MAH-AM水凝胶具有高溶胀率(180%),在15 h后仍能保留15%左右的水分。水凝胶在猪皮上的剪切黏附强度可达到5.575 kPa。双网络超分子水凝胶对大肠杆菌和金黄色葡萄球菌抑菌带宽度分别为0.35~1.5 cm和0.55~1.75 cm,抗菌水凝胶溶血率均在5%以下,且水凝胶浸提液中细胞存活率均在80%以上。

Carboxymethyl-β-cyclodextrin(CM-β-CD) was prepared by dry grinding, and tannic acid(TA) was embedded into the inclusion compound. Maleic anhydride(MAH) and acrylamide(AM) were copolymerized in-situ in chitosan(CS) by free radical reaction, and the inclusion compound was loaded on chitosan. Chitosan and P(MAH-rAM) were ion-coordinated to form TA-CM-β-CD/CS/MAH-AM double-network supramolecular hydrogels. The performance was tested by FT-IR, SEM, electronic universal material tester and biological experiment. The results show that when the mass ratio of TA and CM-β-CD is 1:1(50 mg), the TA-CM-β-CD/CS/MAH-AM hydrogel has a high swelling rate(180%) and can still retain about 15% of water after 15 h. The shear adhesion strength of hydrogel on pig skin can reach 5.575 kPa. The antibacterial bands of network supramolecular hydrogels to Escherichia coli and Staphylococcus aureus are 0.35~1.5 cm and 0.55~1.75 cm, respectively. The hemolysis rate of antibacterial hydrogels is below 5%, and the cell survival rate in hydrogel extracts is above 80%.