血浆miR-let-7e-5p、LncRNA NEAT1表达水平与2型糖尿病视网膜病变的关系

The relationship between expression levels of plasma miR-let-7e-5p and LncRNA NEAT1 and type 2 diabetic retinopathy

目的检测2型糖尿病(type 2 diabetes mellitus,T2DM)患者血浆中微小RNA-let-7e-5p(minute RNA-let-7e-5p,miR-let-7e-5p)和长链非编码核富含丰富的转录本1(lncRNA nuclear-enriched abundant transcript 1,LncRNA NEAT1)的表达水平,并讨论其与糖尿病视网膜病变(diabetic retinopathy,DR)的关系。方法从我院选取收治的T2DM患者126例纳入研究,根据有无DR情况分为84例DR组和42例非DR组,根据DR分级标准分为31例非增生型DR(non-proliferative DR,NPDR)和53例增生型DR(proliferative DR,PDR)。采用荧光定量聚分酶链反应(polymerase chain reaction,PCR)技术检测T2DM患者血浆中miR-let-7e-5p和LncRNA NEAT1的表达水平,比较各组间miR-let-7e-5p和LncRNA NEAT1表达水平的差异;Pearson相关系数分析DR组患者血浆miR-let-7e-5p和LncRNA NEAT1表达水平的相关性;ROC曲线检测miR-let-7e-5p、LncRNA NEAT1对DR的诊断价值。结果非DR患者相比,PDR和NPDR患者的血浆miR-let-7e-5p表达水平降低,LncRNA NEAT1表达水平增加(P<0.05);并且与NPDR组相比,PDR组的血浆miR-let-7e-5p表达水平降低,LncRNA NEAT1表达水平升高(P<0.05);DR患者血浆miR-let-7e-5p与LncRNA NEAT1呈负相关(r=-0.534,P<0.05);miR-let-7e-5p诊断DR的AUC为0.856(95%CI:0.789~0.924),敏感度为77.38%,特异度为80.95%;LncRNA NEAT1诊断DR的AUC为0.844(95%CI:0.778~0.910),敏感度为69.05%,特异度为95.24%;两者联合诊断DR的AUC为0.935(95%CI:0.891~0.978),敏感度为88.10%,特异度为92.86%,两者联合对DR患者具有较高的诊断能力。结论miR-let-7e-5p和LncRNA NEAT1与T2DM患者DR的发生和进展有关,可能作为诊断DR发生的一种潜在生物标志物,且两者联合的诊断能力更佳。

Objective To detect the expression levels of minute RNA-let-7e-5p(miR-let-7e-5p-p)and long-chain non-coding nuclear-rich transcript 1(LncRNA NEAT1)in the plasma of patients with type 2 diabetes(T2DM),and to discuss its relationship with diabetic retinopathy(DR).Methods In total,126 T2DM patients admitted to our hospital were selected and included in the study.They were divided into DR group(n=84)and non-DR group(n=42)according to presence of DR,and non-proliferative DR(NPDR)group(n=31)and proliferative DR(PDR)group(n=53)according to DR classification criteria.The expression levels of miR-let-7e-5p and LncRNA NEAT1 in plasma of T2DM patients were detected by fluorescent quantitative polymerase chain reaction(PCR),and the difference in miR-let-7e-5p and LncRNA NEAT1 expression levels were compared between the groups.Pearson correlation coefficient analysis was used to analyze the correlation of miR-let-7e-5p and LncRNA NEAT1 in the DR group,and diagnostic value of miR-let-7e-5p and LncRNA NEAT1 in DR was detected by receiver operating characteristic(ROC)curve.Results Compared with the non-DR patients,both PDR and NPDR patients had reduced expression levels of plasma miR-let-7e-5p,and increased expression level of LncRNA NEAT1(P<0.05).Compared with the NPDR group,plasma miR-let-7e-5p expression levels were decreased in the PDR group,while LncRNA NEAT1 expression level was increased(P<0.05).Plasma miR-let-7e-5p was inversely associated with LncRNA NEAT1 in DR patients(r=-0.534,P<0.05).The area under the ROC curve(AUC)of the miR-let-7e-5p in the diagnosis of DR was 0.856(95%CI:0.789-0.924),the sensitivity was 77.38%,and the specificity was 80.95%.The AUC of LncRNA NEAT1 in the diagnosis of DR was 0.844(95%CI:0.778-0.910),with the sensitivity of 69.05%and the specificity of 95.24%.The AUC of the combined detection of the two in the diagnosis of DR was 0.935(95%CI:0.891-0.978),with the sensitivity of 88.10%and the specificity of 92.86%.The combined detection had a high diagnostic efficacy for DR patients.Conclusion miR-let-7e-5p and LncRNA NEAT1 are related to the occurrence and progression of DR in T2DM patients,and may serve as potential biomarkers for the diagnosis of DR,and the combination has better diagnostic efficacy.