基于苄基三乙基溴化铵和正辛酸的低共熔溶剂的制备及其在分散液液微萃取中的应用

Preparation of A Deep Eutectic Solvent Based on Benzyltriethylammonium Bromide and Octanoic Acid and Its Application in Dispersion Liquid-liquid Microextraction

以苄基三乙基溴化铵和正辛酸制备出新型低共熔溶剂,并作为萃取溶剂建立了漩涡辅助分散液-液微萃取结合高效液相色谱测定3种苏丹红染料的方法。对影响萃取效率的各种参数,包括低共熔溶剂的组成、萃取时间、萃取剂用量、溶液pH值和盐浓度等进行了优化。结果表明最佳萃取条件为:以苄基三乙基溴化铵与正辛酸物质的量比1∶2制备出的低共熔溶剂为萃取溶剂,萃取剂用量为50μL,萃漩涡时间为2 min,溶液pH 7,盐浓度为0 mg/mL。在最优化条件下,3种苏丹红染料在8~1000 ng/mL范围内线性关系良好,相关系数均大于0.9956,检出限和定量限范围分别为2~2.5 ng/mL和6~8 ng/mL,对果汁和环境水样进行加标实验,回收率为82.3%~111.5%,相对平均偏差范围为0.5%~7.2%。方法简单、快速、绿色环保,可用于真实样品中苏丹红染料的分析检测。

A new hydrophobic deep eutectic solvent(DES),formed from benzyltriethylammonium bromide and octanoic acid,was used as an extraction solvent in vortex-assisted dispersive liquid-liquid microextraction combined with high-performance liquid chromatography for the preconcentration and detection of three Sudan dyes from food samples.Factors affecting the extraction efficiency including the type and volume of extraction solvent,extraction time,solution pH and salt concentration were optimized.The optimum extraction conditions were:volume of extraction solvent was 50μL,vortex time was 2 min,solution pH was 7,salt concentration was 0 mg/mL.Under the optimized condition,a linear range was in the range of 8~1000 ng/mL with a correlation coefficient higher than 0.9956,limits of detection ranged from 2 to 2.5 ng/mL,and limits of quantification from 6 to 8 ng/mL.The food samples were spiked with 20,50,and 100 ng/mL of Sudan dyes,and the extraction recoveries ranged from 82.3% to 111.5% with RSDs ranging from 0.5% to 7.2%.The developed method is simple,quick,and environmentally friendly,which can be applied to detect the Sudan dyes in real food samples.