咖啡酸螯合锌的体外生物活性研究

Biological Activity Research of Caffeic Acid Chelated Zinc in Vitro

以硫酸锌为对照,在模拟胃液和肠液环境下,测定咖啡酸螯合锌中锌离子的吸收率;在模拟肠液环境下,测定咖啡酸螯合锌中锌离子的透过率。以咖啡酸为对照,采用微量二倍梯度稀释法测定咖啡酸螯合锌对猪源和禽源大肠杆菌、沙门菌的体外最低抑菌浓度(MIC)值。咖啡酸螯合锌在模拟胃、肠环境下的锌离子吸收率显著(P<0.05)高于硫酸锌;在模拟肠环境下的锌离子透过率显著(P<0.05)高于硫酸锌。咖啡酸螯合锌对猪源大肠杆菌、沙门菌的体外抑菌MIC值均低于咖啡酸,对不同动物来源的病原菌MIC值有一定差异。咖啡酸螯合锌模拟胃肠吸收率优于硫酸锌,抗菌活性优于咖啡酸,为开发一种新型的锌源和替抗产品的研究提供基础。

The absorption rate of zinc ions in caffeic acid chelated zinc is determined under simulated gastric juice and intestinal juice environment,and the transmission rate of zinc ions in caffeic acid chelated zinc is determined under simulated intestinal fluid environment,with zinc sulfate as the control.With caffeic acid as the control,the minimum inhibitory concentration(MIC)of caffeic acid chelated zinc against Escherichia coli and Salmonella from pigs and birds in vitro is determined by micro double gradient dilution method.The absorption rate of caffeic acid chelated zinc is significantly higher(P<0.05)than that of zinc sulfate under simulated gastric and intestinal environment.The permeability of zinc ions in simulated intestinal environment is significantly higher than that of zinc sulfate(P<0.05).The MIC value of zinc caffeic acid chelate against Escherichia coli and Salmonella from pigs in vitro is lower than that of caffeic acid,and the MIC value against pathogenic bacteria from different animal sources is somewhat different.The simulated gastrointestinal absorption rate of caffeic acid chelated zinc is better than that of zinc sulfate,and its antibacterial activity is better than that of caffeic acid,which provides a basis for the development of a new type of zinc source and alternative anti drug product.