负载氯己定的埃洛石纳米管对牙本质粘结强度的影响

Effect of chlorhexidine-loaded halloysite nanotube on human dentin bond strength

目的:探讨将负载氯己定的埃洛石纳米管掺入全酸蚀粘接剂ONE-STEP及自酸蚀粘接剂XENOⅤ后对牙本质粘结强度的影响。方法:将120颗离体牙分为10组,根据粘接剂XE、OS以及掺入负载氯己定(chlorhexidine,CHX)的埃洛石纳米管(halloysite nanotube,HNT)质量体积比5%、10%、15%分组,分别为XE对照组(A1组)、XE实验对照组(A2组)、5%CHX/HNT-XE组(A3组)、10%CHX/HNT-XE组(A4组)、15%CHX/HNT-XE组(A5组)、OS对照组(B1组)、OS实验对照组(B2组)、5%CHX/HNT-OS组(B3组)、10%CHX/HNT-OS组(B4组)和15%CHX/HNT-OS组(B5组)。树脂充填24 h后,测试拉伸剪切强度(tensile shear strength,TSS)值。电镜扫描,观察粘结界面嵌合层的形态。采用SPSS 19.0软件包对同一粘接剂各组以单因素方差分析TSS值,不同粘接剂及不同质量比各组以双因素方差分析TSS值。结果:自酸蚀XE多组中,5%CHX/HNT组的TSS值最高;全酸蚀OS组中,10%CHX/HNT组的TSS值最高(P<0.05)。不同品牌粘接剂对TSS无显著影响,而CHX/HNT质量体积比对TSS有显著影响。结论:将适合浓度的CHX/HNT掺入自酸蚀粘接剂XENOⅤ和全酸蚀粘接剂ONE-STEP中,可增加牙本质粘结强度。

PURPOSE:To evaluate the effect of chlorhexidine-loaded halloysite aluminosilicate clay nanotube(HNT)incorporation into a two-step etch-and-rinse(ONE-STEP)and a self-etch(XENOⅤ)adhesive on human dentin tensile shear strength(TSS).METHODS:Ten groups(n=12)were prepared according to the adhesive system and amount of CHX/HNT incorporated(5%,10%,15%)as follows:XE control(A1),XE experimental control(A2),5%CHX/HNT-XE(A3),10%CHX/HNT-XE(A4),15%CHX/HNT-XE(A5),OS control(B1),OS experimental control(B2),5%CHX/HNTOS(B3),10%CHX/HNT-OS(B4),15%CHX/HNT-OS(B5).TSS testing was performed after 24 h.Scanning electron microscopy of the resin-dentin interface of selected specimens was carried out.One-way ANOVA was used to evaluate the TSS date in XE groups and OS groups.Two-way ANOVA was used to evaluate the effect of the adhesive system and the CHX/HNT content on dentin TSS with SPSS 19.0 software package.RESULTS:The TSS date of 5%CHX/HNT-XE was significantly higher than the other groups.The TSS date of 10%CHX/HNT-OS was significantly higher than the other groups(P<0.05).No significant difference was detected between adhesive systems and dentin TSS.significant difference was found between CHX/HNT content and dentin TSS.CONCLUSIONS:CHX/HNT addition into dentin adhesive increased bond strength to dentin.