大鼠牙齿移动过程中Piezo1对压力侧牙周组织中RIP3及MLKL的影响

Effect of Piezo1 on RIP3 and MLKL in periodontal tissue of the pressure side during tooth movement in rats

目的 探究大鼠牙移动过程中,在不同时间点Piezo1对压力侧牙周组织中坏死性凋亡标志物受体相互作用蛋白3(receptor-interacting protein 3,RIP3)、混合谱系激酶结构域样蛋白(mixed lineage kinase domain-like protein, MLKL)的影响。方法 选用雄性SD大鼠75只,随机分为对照组(A组),加力组(B组),加力+抑制剂组(C组)。构建大鼠上颌第一磨牙移动的正畸模型,通过游标卡尺测量正畸牙向近中移动的距离,应用苏木精-伊红(hematoxylin-eosin, HE)染色观察压力侧牙周组织变化,免疫组化法检测RIP3、MLKL表达量的变化。结果 与A组相比较,B组和C组牙周组织中RIP3、MLKL表达均呈先增加后下降的趋势(P<0.05),其中RIP3表达量增加至5 d时达到高峰,MLKL表达量增加至3 d时达到高峰;与B组相比较,C组牙周组织中RIP3、MLKL表达整体下降(P<0.05),表达趋势变化与B组一致。结论 Piezo1通道在正畸牙齿移动过程中可能起着机械转导的作用,阻断Piezo1通道可下调压力侧牙周组织中RIP3和MLKL的表达,减少坏死性凋亡的发生,减慢正畸牙齿移动的速度。

Objective To observe the influence of Piezo1 on the necroptosis markers receptor-interacting protein 3(RIP3) and mixed lineage kinase domain-like protein(MLKL) in pressure-side periodontal tissue at various time points during tooth movement in rats. Methods Seventy-five male SD rats were randomly distributed into control group(group A), force group(group B) and force + inhibitor group(group C). The orthodontic model of the maxillary first molar was constructed in rats. The moving distance of the orthodontic tooth was measured with vernier calipers. Hematoxylin-eosin(HE) staining was used to observe changes of pressure-side periodontal tissue, and changes of RIP3 and MLKL were tested by immunohistochemical staining. Results Compared with group A, expression levels of RIP3 and MLKL in periodontal tissue in group B and C increased firstly and then fell(P<0.05), and the expression of RIP3 increased to the peak on the 5 th day, MLKL on the 3 rd day. Compared with group B, the expression of RIP3 and MLKL in periodontal tissue in group C were decreased as a whole(P<0.05), and the expression trend was consistent with group B. Conclusion Piezo1 channel may play a role in the mechanotransduction process of orthodontic tooth movement. Blocking the function of Piezo1 channel may reduce the expression of RIP3 and MLKL in pressure-side periodontal tissue, reduce the occurrence of necroptosis and the speed of orthodontic tooth movement.