载有卵磷脂的纳米纤维膜的制备及其抗细胞黏附性能评价

Preparation of nanofiber membranes loaded with phosphatidylcholine and evaluation of their cell anti-adhesion properties

借助静电纺丝技术,将不同质量浓度卵磷脂作为功能性添加剂与聚乳酸和聚羟基乙酸共混纺丝,进而探究制备腹腔防粘连膜的可行性。采用场发射扫描电子显微镜、红外光谱、强力仪、接触角测量仪及细胞试验对复合纤维膜的纤维形貌、表面成分、力学性能、亲疏水性、降解性能及抗细胞黏附性等进行表征。结果表明,卵磷脂被成功负载于纤维膜,载有卵磷脂的纤维膜具有良好的亲水性及力学性能,能够保持降解28 d的结构完整性,同时具有良好的抗L-929成纤维细胞黏附性能。由此可见,负载卵磷脂的纳米纤维膜在防粘连领域具有潜在应用前景。

By using electrospinning technology,phosphatidylcholine with different mass concentrations was added to polylactic acid and polyglycolic acid for blending spinning,thereby investigating the feasibility of preparing an anti-adhesion membrane for the peritoneal cavity.The fiber morphology,surface composition,mechanical properties,hydrophilic and hydrophobic properties,degradation properties,and anti-cell adhesion properties of the composite film were characterized by field emission scanning electron microscope,infrared spectrum,strength meter,contact angle meter,and cell experiments.The results show that phosphatidylcholine is successfully loaded into the fiber membrane,and the fiber membrane with phosphatidylcholine has good hydrophilicity and appropriate mechanical properties,and can maintain structural integrity after degradation for 28 days,and has good adhesion resistance to L-929 fibroblasts.It shows that the fiber membrane with phosphatidylcholine has potential application in the field of anti-adhesion.