深共熔溶剂提取杨树桑黄多酚类物质工艺优化及提取物抗氧化活性研究

Optimization of extraction process and examination of antioxidant activities of polyphenols extracted from Sanghuangporus vaninii by using deep eutectic solvent

桑黄是一类应用广泛的药用真菌,桑黄多酚具有抗氧化、抗炎和降糖作用。本研究中的杨树桑黄属于桑黄中重要且能栽培的一种。本研究采用深共熔溶剂(deep eutectic solvent,DES)从栽培杨树桑黄子实体中提取多酚类化合物,考察了不同提取条件对提取率的影响。采用氯化胆碱与尿素组成的DES体系对多酚进行提取,并进一步采用响应面法对提取条件进行优化,获得最佳提取工艺参数为21 min、80℃、料液比1:260 g/mL。在此条件下,多酚的提取率高达(23.17±0.88)mg/g,远高于传统方法(12.45±1.88)mg/g。最优条件提取的多酚显示了很强的DPPH和ABTS的清除能力。由此可见,采用DES法从杨树桑黄子实体中提取酚类化合物比传统方法更有效。

Sanghuangporus spp.are widely used as medicine,and the polyphenols extracted from these fungi possess antioxidant,anti-inflammatory and hypoglycaemic effects.Sanghuangporus vaninii is one of the important and cultivated species.Deep eutectic solvent(DES)was used to extract phenolic compounds from the fruiting bodies of artificially cultivated S.vaninii.The factors affecting extraction yield were investigated.The DES system with choline chloride and urea was used and the extraction conditions were further optimized by response surface methodology.As a result,the optimal extraction parameters were obtained as follows:extraction duration of 21 min,extraction temperature of 80°C,and the material-to-liquid ratio of 1:260 g/mL.The polyphenol yield reached(23.17±0.88)mg/g under these conditions,being much higher as compared with that obtained by the traditional method(12.45±1.88)mg/g.The extracted polyphenols showed excellent scavenging ability against DPPH and ABTS radicals.The DES method of extracting phenolic compounds from the fruiting bodies of S.vaninii was proved to be more effective than the conventional method.