GC-FID法测定消癥丸中莪术酮、藁本内酯和α-香附酮

Determination of curzerenone,ligustilide,and α-cyperone in Xiaozheng Pills by GC-FID

目的建立气相色谱-氢火焰离子化检测器(GC-FID)法同时测定消癥丸中莪术酮、藁本内酯和α-香附酮的方法。方法采用HP-5MS毛细管柱(30 m×0.25 mm×0.25μm),FID检测器:280℃,进样口温度240℃;程序恒温130℃,保持45 min;载气为N_(2),柱体积流量1.5 mL/min,不分流模式;进样量:1μL。结果莪术酮、藁本内酯、α-香附酮分别在1.684~168.400、2.496~249.600、2.180~218.000μg/mL线性关系良好;平均回收率分别为97.98%、99.76%、96.06%,RSD值分别为1.62%、0.89%、1.29%。结论该方法操作简单、重复性好,可为评价消癥丸的质量控制提供依据。

Objective To establish a method for simultaneous determining of curzerenone,ligustilide,andα-cyperone in Xiaozheng Pills by GC-FID method.Methods The separation was carried out on HP-5MS column(30 m×0.25 mm×0.25μm).Detector was FID detector,and the detector temperature was 280℃,and the inlet temperature was 240℃.The program temperature was 130℃for 45 min.The carrier gas was nitrogen,and the flow rate was 1.5 mL/min with splitless inlet.The injection volume was 1μL.Results The linear ranges of curzerenone,ligustilide,andα-cyperone were 1.684—168.400,2.496—249.600,2.180—218.000μg/mL,respectively.The average recoveries of curzerenone,ligustilide,andα-cyperone were 97.98%,99.76%,and 96.06%with RSD values of 1.62%,0.89%,and 1.29%,respectively.Conclusion The established method is simple and reproducible,which can be used for the quality control in Xiaozheng Pills.