中国南瓜BZR基因家族的全基因组鉴定及生物信息学分析

Genome-wide Identification and Bioinformatics Analysis of BZR Gene Family in Pumpkin(Cucurbita moschata Duch.)

油菜素唑抗性因子(BZR)是植物激素油菜素内酯信号转导途径中的关键转录因子,通过激活相关基因的表达,调控植物的生长发育和环境响应。为了解析中国南瓜BZR基因家族的特征,本研究使用生物信息学技术鉴定中国南瓜基因组中BZR基因家族成员并进行理化性质、染色体定位、共线性、系统发育、基因结构、保守基序和顺式作用元件分析。结果显示,中国南瓜基因组中共有8个BZR基因家族成员,理化性质差异不明显;8个BZR基因均匀分布在中国南瓜的染色体上;系统发育分析显示8个BZR蛋白构成两个支系,结合共线性分析表明中国南瓜8个BZR基因可能是基因复制的结果。基因结构和保守基序分析显示中国南瓜的BZR基因之间差异不明显;顺式作用元件分析显示中国南瓜8个BZR基因家族成员可能在中国南瓜油菜素内酯信号转导途径中扮演不一样的角色。研究结果为进一步研究中国南瓜BZR基因家族生物学功能提供参考。

Brassinazole resistant transcription factor(BZR)is a key transcription factor in brassinosteroid(BR)signal transduction in plants.BZR genes activate the expression of related genes to regulate plant growth and development and environmental response.In order to analyze the characteristics of the BZR gene family in pumpkin(Cucurbita moschata Duch.),the BZR gene family members in pumpkin were identified,and the physicochemical properties,chromosomal localization,collinearity relationship,phylogenetic relationship,gene structure,conserved motif and cis-regulatory elements were analyzed and predicted.The eight BZR genes showed similar physicochemical properties and were distributed evenly in the pumpkin genome.Phylogenetic analysis showed that eight BZR proteins were divided into two clades,and eight BZR genes might be the result of gene duplication combined with the result of collinearity analysis.There was no obvious difference in the gene structure and conserved motifs among the eight BZR genes.Cis-regulatory element analysis indicated the eight BZR genes may play different roles in BR signal transduction in pumpkin.Our study provides a reference for further researching the biological functions of the BZR gene family in pumpkin.