青萝卜种质资源耐抽薹性鉴定及亲缘关系分析

Identification of Bolting Tolerance and Genetic Relationship Analysis of Green Radish Germplasm Resources

本研究以64份青萝卜种质为供试材料,通过田间鉴定来综合评价青萝卜种质的耐抽薹性,并利用SSR分子标记技术探究上述种质间的亲缘关系。基本统计分析表明,供试材料7个指标(现蕾期,抽薹期,开花期,薹高,株高,单根重,单株重)间存在显著性差异(P<0.05)。相关性分析表明,现蕾期、抽薹期、开花期之间相关性达到极显著水平(P<0.01),均可用来评价青萝卜种质耐抽薹性,其中现蕾期是最简单、高效的评价指标。采用隶属函数法将64份种质分为3个组,筛选出中等耐抽薹种质40份。田间性状聚类分析表明,64份种质被分为4个类群,第Ⅱ类群和第Ⅳ类群中70%的种质均属于隶属函数法划分的中等耐抽薹等级,说明隶属函数法和聚类分析法均可以有效、可靠地鉴定和评价青萝卜种质的耐抽薹性。用筛选出的11对核心引物对64份青萝卜种质进行PCR扩增,共获得清晰条带54条,平均每对引物扩增条带数为4.91条,多态性条带比率为93.64%。青萝卜种质的遗传相似系数为0.3226~0.9787,平均值为0.6967。UPGMA聚类分析表明,在遗传相似系数为0.636处,可将64份青萝卜种质分为三大类。本研究可为耐抽薹青萝卜种质创新及优良品种选育提供理论依据。

In this study,64 green radish germplasms were used as test materials to comprehensively evaluate the bolting tolerance of green radish germplasms through field identification,and SSR molecular marker technology was used to explore the genetic relationship between the above germplasms.Basic statistical analysis showed 7 indexes(budding date,bolting date,flowing date,weight of sing plant,weight of sing root,stem height,plant height)of the test materials were significant difference(P<0.05).Correlation analysis showed that the correlation between budding stage,bolting stage,and flowering stage were different in highly significant(P<0.01),which can be used to evaluate the bolting tolerance of green radish germplasm,and budding stage is the simplest and most efficient evaluation index.Using membership function method,64 germplasms were divided into 3 groups,and screened 40 germplasms with moderate bolting tolerance.The cluster analysis of field traits showed that 64 germplasms were divided into 4 groups,and 70%of the germplasms in groupⅡand groupⅣbelonged to the medium bolting-tolerant grade divided by the membership function method.It showed that both the membership function method and cluster analysis method can effectively and reliably identify and evaluate the bolting tolerance of green radish germplasm.The 11 pairs of core primers selected were used for PCR amplification of 64 green radish germplasms,and a total of 54 clear bands were obtained,the average number of amplified bands per primer pair was 4.91,and the ratio of polymorphic bands was 93.64%.The genetic similarity coefficient of green radish germplasm was 0.3226~0.9787,with an average value of 0.6967.UPGMA cluster analysis showed that 64 green radish germplasms were divided into three categories at the genetic similarity coefficient of 0.636.This study can provide a theoretical basis for the innovation of bolting-resistant green radish germplasm and the breeding of excellent varieties.