利用二醛木聚糖一锅法制备纳米银抗菌水凝胶

One-pot preparation of antibacterial nanosilver hydrogels from dialdehyde xylan

利用二醛木聚糖(DAX)可与羧甲基壳聚糖(CMCS)及银氨溶液同时反应的特性,以聚乙烯醇(PVA)为结构稳定剂,一锅法制备了具有优异抗菌性能的CMCS/PVA纳米银抗菌水凝胶。采用FTIR、TGA、TEM、XRD和SEM对DAX,纳米银粒子(SNPs)和水凝胶的形貌、结构进行了表征。结果表明,DAX在整个反应中起到了双功能作用,成功地与CMCS和PVA发生反应,共同构成了双网络结构的水凝胶,提高了水凝胶的热稳定性及力学性能。DAX在室温条件下能快速还原出SNPs,SNPs粒径在20~80 nm之间,具有较好的分散性。SNPs的参与使CMCS/PVA水凝胶对大肠杆菌和金色葡萄球菌抗菌效果显著提高。此外,制备的水凝胶具有良好的细胞相容性,其对L929细胞的细胞相对存活率都在95%以上。

Carboxymethyl chitosan(CMCS)/polyvinyl alcohol(PVA) nanosilver antibacterial hydrogels were successfully synthesized by one-pot reaction of dialdehyde xylan(DAX) with CMCS and silver ammonia solution using PVA as structural stabilizer. The morphology and structure of DAX, silver nanoparticles(SNPs) and hydrogels obtained were characterized by FTIR, TGA, TEM, XRD and SEM. The results showed that DAX, playing a dual function in the whole process, successfully reacted with CMCS and PVA forming a double network, which improved the thermal stability and mechanical properties of hydrogels. Meanwhile, SNPs, produced by the quick reduction reaction between DAX and silver ammonia at room temperature, showed a particle size distribution between 20 and 80 nm, good dispersity, and significant enhancement of the antibacterial efficacy of CMCS/PVA hydrogels against Escherichia coli and Staphylococcus aureus. In addition, the hydrogels exhibited good cytocompatibility with the cell relative survival rate of L929 cells cultured in medium containing hydrogel above 95%.