翘芩清肺剂对肺炎克雷伯菌和金黄色葡萄球菌的抑菌效果初探

Preliminary study on bacteriostatic-effect of Qiaoqinqingfei on Klebsiella pneumoniae and Staphylococcus aureus

目的基于血清药理学评估翘芩清肺剂对肺炎克雷伯菌和金黄色葡萄球菌的体外抑菌作用。方法新西兰兔(每组7例)胃饲翘芩清肺剂后,分别于第1天0.5 h、1 h、2 h、3 h和2 d、3 d、4 d、5 d、6 d、7 d、8 d、9 d、10 d连续10 d抽取血液样本,制备含药血清,以CCK-8法分别观察翘芩清肺剂对肺炎克雷伯菌标准菌株ATCC700603、金黄色葡萄球菌标准菌株ATCC25923的抑菌效果。结果10 d翘芩清肺剂对肺炎克雷伯菌抑菌率(39.29±6.50)%,2例有效抑菌率>50%,差异无统计学意义;金黄色葡萄球菌抑菌率为(97.86±0.60)%,7例有效抑菌率>50%,差异具有统计学意义(P<0.05)。结论翘芩清肺剂对金黄色葡萄球菌的抑菌效果较肺炎克雷伯菌佳。

Objective Serum pharmacology was used to evaluate the bacteriostatic effect of Qiaoqinqingfei on Klebsiella pneumoniae and Staphylococcus aureus in vitro.Methods Rabbits were given with Qiaoqinqingfei by gavage.Blood samples were taken at 0.5 h,1 h,2 h,3 h on the first day and at 2 d,3 d,4 d,5 d,6 d,7 d,8 d,9 d and 10 d for 10 consecutive days,respectively.Serum containing drugs were prepared,and the antibacterial effect of Qiaoqinqingfei on Staphylococcus aureus ATCC25923 and Klebsiella pneumoniae ATCC700603 was observed by CCK-8 method.Results The bacteriostatic rate of Qiaoqinqingfei against Staphylococcus aureus was 97.86±0.60 at 10 day,the effective bacteriostatic rate of 7 cases>50,which was statistically significant(P<0.05).The bacteriostatic rate of Klebsiella pneumoniae was 39.29±6.50,and the effective bacteriostatic rate of 2 cases>50 at 10 day,which was not statistically significant.Conclusion The antibacterial effect of Qiaoqinqingfei on Staphylococcus aureus was better than that of Klebsiella pneumonia.