大鼠急性阻塞性睡眠呼吸暂停模型下的左侧肾神经活性变化

The change of left renal sympathetic nerve activity on acute obstructive sleep apnea rats

目的建立稳定的大鼠肾神经放电记录的方法学及探讨急性阻塞性睡眠呼吸暂停模型下的左侧肾交感神经活性(RSNA)变化.方法成年雄性SD大鼠10只,麻醉后气管插管,反复间歇夹闭气管导管20 s,同步记录大鼠RSNA、心率和动脉血压的变化.结果所有大鼠均在实验中记录到稳定的RSNA、心率及血压图形,并完成了整个实验过程.与闭气前20 s相比,闭气期间:①RS N A的频率从(0.40±0.26)次增加到(9.30±3.92)次(P<0.05);②RSNA的电压从(0.02±0.00)mV增加到(0.03±0.01)mV(P<0.01);③RR间期从(208.48±15.30)m s增加到(224.00±49.75)m s(P<0.05);④血压明显从(122.05±9.82)m m H g下降为(109.71±7.51)m m H g(P<0.01).结论闭气期间RSNA明显增强,RR间期下降,血压明显下降.

Objective To establish a stable method for recording renal sympathetic nerve activity(RSNA) in rats and to investigate the changes of RSNA in acute obstructive sleep apnea model. Methods Ten adult male SD rats were intubated after anesthesia and repeatedly clipped for 20 s. The changes of RSNA, heart rate and arterial blood pressure were recorded synchronously. Results Stable patterns of RSNA,heart rate and blood pressure were recorded in all rats during the whole experiment. Compared with 20 s before air retention,(1) Frequency of RSNA increased from(0.40 ± 0.26) times to(9.30 ± 3.92) times s during air retention(P<0.05);(2) The voltage of RSNA increased significantly from(0.02 ± 0.00) mV to(0.03 ± 0.01) mV(P<0.01);(3) RR interval increased from(208.36 ± 15.30) ms to(224.00 ± 49.75) ms(P<0.05);(4) Blood pressure decreased significantly from(122.05 ± 9.82) mmHg to(109.71 ± 7.51) mmHg(P<0.01). Conclusion RSNA is significantly enhanced during air retention, lower RR interval and lower blood pressure.