海参药材质量标准提升的研究

Research on improving the quality standard of sea cucumber

目的 制定山东省地方习用药材海参的质量标准。方法 参照《中国药典》(2020版)相关方法对海参药材进行性状和薄层色谱(TLC)鉴别研究,测定了水分、酸不溶性灰分及浸出物的含量;通过硫酸苯酚法以葡萄糖为对照品测定海参多糖的含量;采用高效液相色谱法(HPLC)对海参药材的特征图谱进行研究。结果 不同批次海参药材的性状特征较一致;TLC斑点清晰,重复性好;海参药材中水分的质量分数为6.1%~10.8%,酸不溶性灰分的质量分数为0.08%~1.44%,浸出物的质量分数范围为33.6%~51.7%,以葡萄糖计海参多糖的质量分数范围为0.52%~0.85%;供试品色谱图中呈现的5个特征峰与对照药材参照物色谱图中的5个特征峰的保留时间相对应。结论 建立的定性及定量方法操作简便、准确、稳定,可用于海参药材的质量控制。

Objective To establish the quality standards of the local medicinal sea cucumber in Shandong Province. Methods According to the relevant methods of the Chinese Pharmacopoeia(2020 edition), the properties and thin-layer chromatography(TLC) identification of sea cucumber medicinal materials were studied. The contents of water, acid-insoluble ash and extracts were determined. The content of sea cucumber polysaccharides was determined by ultraviolet-visible spectrophotometry. The characteristic chromatogram of sea cucumber medicinal materials was studied using the HPLC method. Results The traits of different batches of sea cucumber medicinal materials were relatively consistent. TLC spots were clear and reproducible. The water content in sea cucumber medicinal materials was 6.1%–10.8%, the acid-insoluble ash content was 0.08%–1.44%, and the extract content was 33.6%–51.7%. Calculated by glucose, sea cucumber polysaccharide content was 0.52%–0.85%. The retention time of the five characteristic peaks in the chromatogram of the test samples corresponds to that of the reference medicinal material. Conclusion The method established was simple, accurate and stable, and could be used for the quality control of sea cucumber medicinal materials.