辣椒素抑制自发性高血压大鼠脑基底动脉平滑肌细胞的增殖和迁移

Capsaicin inhibits proliferation and migration of cerebral basilar artery smooth muscle cells in spontaneously hypertensive rats

目的研究辣椒素(CAP)是否可以改善自发性高血压大鼠(SHR)脑基底动脉平滑肌细胞(BASMC)的增殖迁移。方法体外培养SHR及Wistar京都(WKY)大鼠原代BASMC,随机均分为对照组(WKY组)、SHR组、CAP处理的SHR组。通过CCK-8法选取CAP处理浓度;Transwell^(TM)小室实验和划痕实验检测各组BASMC的迁移能力;免疫荧光细胞化学染色检测BASMC中的骨桥蛋白(OPN)、增殖细胞核抗原(PCNA)的表达和分布;Western blot法检测BASMC的OPN、PCNA蛋白水平。结果与WKY组相比,SHR组BASMC增殖和迁移能力增强,经CAP处理后,细胞增殖和迁移能力降低;OPN表达在BASMC胞质和胞核,PCNA主要表达在胞核,与WKY组相比,SHR组OPN、PCNA表达及蛋白水平增强,经CAP处理后,其OPN、PCNA表达及蛋白水平明显降低。结论CAP可降低SHR来源的BASMC的增殖和迁移。

Objective To investigate whether capsaicin(CAP)can improve the proliferation and migration of cerebral basilar artery smooth muscle cells(BASMCs)in spontaneously hypertensive rats(SHR).Methods Primary BASMCs of SHR and Wistar-Kyoto(WKY)rats were cultured in vitro,randomly divided into control group(WKY group),SHR group and capsaicin treatment group(CAP group).The intervention concentration of CAP was determined by CCK-8 assay;Transwell^(TM) chamber assay and scratch test were used to detect the migration ability of BASMCs;the expression and distribution of osteopontin(OPN)and proliferating cell nuclear antigen(PCNA)in BASMCs were detected by immunofluorescence assay,and Western blot analysis was used to detect the protein levels of OPN and PCNA in BASMCs.Results Compared with WKY group,the proliferation and migration ability of BASMC in SHR group were enhanced,while the CAP treatment undermined the proliferation and migration of BASMCs.OPN was expressed in the cytoplasm and nucleus of BASMCs,while PCNA was mainly expressed in the nuclei.Compared with WKY group,the expression and protein level of OPN and PCNA were increased in SHR group,and decreased significantly after CAP treatment.Conclusion Capsaicin can reduce the proliferation and migration of SHR derived BASMCs.