不停跳冠脉搭桥术后差异基因的生信分析

Bioinformatics analysis of differential expression genes after off-pump coronary artery bypass grafting

目的通过生信分析探讨不停跳冠脉搭桥(OPCAB)术后基因变化,并筛选关键(Hub)基因.方法通过纳入数据集GSE12485(心肌)和GSE4386(心房),使用R包对OPCAB术前术后心脏组织进行差异分析,筛选差异基因(DEGs).两组DEGs交集得到共同DEGs进一步分析,然后进行GO和KEGG功能富集分析.最后使用STRING进行蛋白质-蛋白质相互作用(PPI)分析,通过Cytoscape软件中cytoHubba的Degree算法筛选Hub基因.结果共获得36个共同上调DEGs.GO和KEGG分析结果显示,OPCAB术后主要与炎症反应相关.PPI网络分析,并得到4个Hub基因.结论炎症反应在OPCAB术后起重要作用,而4个Hub基因均具有重要的价值,将为后续研究提供依据.

Objective To investigate gene changes after off-pump coronary artery bypass grafting(OPCAB) by bioinformatics analysis, and to screen Hub genes.Methods By including the datasets GSE12485(myocardium) and GSE4386(atrium),the R package was used to analyze the differences in the preoperative and postoperative cardiac tissues of OPCAB to screen for differential genes(DEGs).The intersection of the two groups of DEGs was obtained with the common DEGs for further analysis.Then GO and KEGG functional enrichment analysis was performed.Finally, STRING was used for protein-protein interaction(PPI) analysis, and Hub genes were screened by Degree algorithm of cytoHubba with Cytoscape software. Results A total of 36 co-upregulated DEGs were obtained.GO and KEGG analysis showed that OPCAB was mainly associated with inflammation.PPI network analysis was performed, and four Hub genes with the highest score were obtained. Conclusion Inflammation plays an important role after OPCAB.All the four Hub genes are of great value, which will provide a basis for subsequent research.